CANINE

热疗在治疗肿瘤方面有一定的作用，但应用热疗的最佳方式是 不是很清楚。这个项目的目的是量化一个常量的生理效应。 热疗总剂量，但使用两种不同的处方进行分级。这场审判源于我们之前的 研究表明，加热总持续时间与肿瘤控制时间长短成反比。这 研究结果表明，与高温症相关的生理变化对治疗有影响。 结果表明，较大的热剂量可能与氧合的有害变化有关。如果 热疗的使用有待优化，热疗相关的生理变化很重要 理所当然地理解这一点。这项研究将在患有自发性软组织肉瘤的狗身上进行。他们 除了接受热疗外，还将接受放射治疗。狗将被随机分成粗略的或 精细分级的热疗处方。将会测量血液灌流，氧合， 治疗前和治疗期间多次的血管生成、间质压力和基因表达 评估加热的生理影响的治疗。将使用坏死和细胞凋亡的测量方法 以获得有关两种分割方案对肿瘤的相对影响的信息。它是 预计更细微的分割热疗将与更有利的 与粗大相比，生理参数的变化和细胞凋亡和坏死的增加 分阶段的协议。从这份报告中获得的信息将有助于优化 热疗被用于治疗人类癌症和设计未来的热疗试验。

Hyperthermia has a role in the treatment of tumors but the optinal manner in which to apply heat treatment is not well understood. The purpose of this project is to quantify the physiologic effects of a constant hyperthermia total dose but fractionated using two different prescriptions. This trial arose from our previous work showing an inverse relationship between total duration of heating and length of tumor control. This finding suggests that physiologic changes associated with hypertermia have an influence on treatment outcome and that large thermal doses may be associated with deleterious changes in oxygenation. If hyperthermia use is to be optimized it is important that the physiologic changes associated with hyperthermia frationation be understood. This study will be performed in dogs with spontaneous soft tisue sarcoma. They will receive radiation therapy in addition to hyperthermia. Dogs will be randomized to either a coarsely or finely fractionated hyperthermia prescription. There will be measurements of perfusion, oxygenation, angiogenesis, interstitial pressure and gene expression before treatment and at multiple times during treatment to assess the physiologic effects of heating. Measurements of necrosis and apoptosis will be used to obtain some information on the relative effect of the two fractionation schemes on the tumor. It is anticipated that the more finely fractionated hyperthermia treatment will be associated with more favorable changes in physiologic parameters and increased apoptosis and necrosis compaed to the coarsely fractionated protocol. The information gained from this tiral will be helpful in optimizing the manner in which hyperthermia is used for the treatment of human cancer and for design of future hyperthemria trials.