EFFECTORS OF RAPID HOMEOSTATIC RESPONSES OF ENDOPLASMIC RETICULUM CHOLESTEROL

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 内质网（ER）的胆固醇含量和嵌入其中的3-羟基-3-甲基戊二酰-CoA还原酶（HMGR）的活性在数分钟内对质膜胆固醇水平的变化做出稳态响应。我们现在已经研究了固醇调节元件结合蛋白（SREBP）依赖的基因表达，侧链氧化固醇生物合成，和胆固醇前体在ER胆固醇水平和HMGR活性的短期调节中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The cholesterol content of the endoplasmic reticulum (ER) and the activity of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) imbedded therein respond homeostatically within minutes to changes in the level of plasma membrane cholesterol. We have now examined the roles of sterol regulatory element-binding protein (SREBP)-dependent gene expression, side chain oxysterol biosynthesis, and cholesterol precursors in the short term regulation of ER cholesterol levels and HMGR activity.