PROTEOMIC ANALYSIS OF FOLLICULAR FLUID AND SERA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 莫利实验室正在进行无标记蛋白质组学研究，旨在确定体外受精(IVF)成功的预测因素。补体级联对动物早期妊娠发育至关重要，但补体蛋白在体外受精成功中的作用尚不清楚。我们推测卵泡微环境中的补体活性影响卵母细胞的能力和体外受精的结果。将对正在接受体外受精的患者的血清和卵泡液样本进行分析，以确定成功进行试管受精的潜在生物标志物。蛋白质组学分析揭示了妊娠患者卵泡液中补体蛋白的特征。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Moley lab is undertaking label free proteomics studies that are geared toward determining predictive factors for successful in vitro fertilization (IVF). The complement cascade is critical for early pregnancy development in animals, but the role of complement proteins in success of IVF is unknown. We speculate that complement activity in the follicular microenvironment affects oocyte competence and IVF outcomes. Serum and follicular fluid samples will be analyzed from patients who are undergoing IVF for the purpose of identifying potential biomarkers for successful IVF outcome. Proteomic analysis reveals a signature of complement proteins in the follicular fluid of patients achieving pregnancy.