Self Healing Biomaterials

描述（由申请人提供）：机械失效的原位修复仍然是生物材料研究的一个基本上未经审查的领域。材料科学的一个新兴领域是“自修复材料”（SHM），它使用各种嵌入式化学物质来检测和修复微裂纹，然后再合并成传播的裂纹。该R21提案研究了将生物相容性，无毒SHM技术纳入最简单的承重生物材料配方之一的可行性-双组分丙烯酸骨水泥-最简单的SHM技术-分散在催化剂嵌入的聚合物基质中的微囊化愈合剂。该研究包括四个具体目标。目的1：采用乳化油/水界面聚合法制备微胶囊化氰基丙烯酸烷基酯愈合剂。目的2：将微囊化愈合剂掺入双组分PMMA基质中。目的3：表征自愈合骨水泥的机械性能和断裂力学。目标4：通过在小鼠成纤维细胞培养物中进行浸提介质洗脱试验，以及通过在人成骨细胞培养物中放置的骨水泥样品上进行细胞生长，对自愈合骨水泥进行细胞毒性试验。 公共卫生相关性：所有循环加载生物材料最可能的失效模式是由微裂纹累积引起的疲劳失效。本研究的长期目标是开发具有修复微裂纹水平失效的能力的复合生物材料，从而延长循环加载生物材料的使用寿命。该项目专门测试了使用丙烯酸骨水泥作为模型生物材料的概念可行性。

DESCRIPTION (provided by applicant): In situ repair of mechanical failure remains a largely unexamined area of study in biomaterials. A newly emerging area of materials science is "Self-Healing Materials" (SHM) that uses a variety of embedded chemistries to detect and repair microcracks in situ before they coalesce into propagating cracks. This R21 proposal examines the feasibility of incorporating biocompatible, non-toxic SHM technology into one of the simplest load-bearing biomaterial formulations -- two component acrylic bone cement -- with the most straightforward SHM technology -- microencapsulated healing agent dispersed in a catalyst-embedded polymer matrix. The study consists of four specific aims. Aim 1: Fabrication of microencapsulated alkyl cyanoacrylate healing agent using emulsified oil in water interfacial polymerization. Aim 2: Incorporation of the icroencapsulated healing agent into the two-component PMMA matrix. Aim 3: Characterization of self-healing bone cement mechanical properties and fracture mechanics. Aim 4: Cytotoxicity testing of self-healing bone cement by elution testing of extraction media in mouse fibroblast culture, and by cell ongrowth onto samples of bone cement placed in cultures of human osteoblasts. PUBLIC HEALTH RELEVANCE: The most likely mode of failure of all cyclically loaded biomaterials is fatigue failure resulting from the accumulation of microcracks. The long-term goal of this study is to develop composite biomaterials that have the capacity to repair failure at the microcrack level and thus prolong the use life of cyclically loaded biomaterials. This project specifically tests out the concept feasibility using acrylic bone cement as a model biomaterial.