Structural Genomics on Membrane Proteins

（整个过程中的限制性愿望） 在此输入文本，它是应用程序的新摘要信息。本节必须是否 超过30行的文本。 该提案的总体目标是加快收购结构性 通过应用结构基因组学方法获得膜蛋白的信息 由一组专家调查员的集体经验提供信息。我们有 成立了纽约膜蛋白结构协会（NYCOMPS）， 为实现这一目标而共同努力。作为PSI-Biology的组成部分，NYCOMPS将 由五个机构的十名首席研究员组成。我们的结构管道 确定将通过已知序列的生物信息学分析来选择靶， 继续进行重组DNA克隆，在细菌或真核细胞中表达蛋白质， 和蛋白质纯化在中等高通量，然后继续确定 X射线晶体学结构。我们在纽约的蛋白质生产设施 结构生物学中心（NYSBC）通过中等规模的纯化处理靶标 并将成功的候选者分发给参与实验室，以便扩大规模 和结晶。结构的最小功能分析将由 计算和常规实验生物化学。将确定目标 通过生物界的提名，包括附属的NYCOMPS 成员，并从NYCOMPS生物主题，其中涉及阐明的 能量稳态和代谢的膜蛋白宇宙和结构研究 紊乱技术开发将仅限于管道的直接改进 效率将对该项目进行管理，以优化产出并有效整合 与PSI-Biology网络和其他膜蛋白结构的努力。

(Restricted aspirations throughout) Enter the text here that is the new abstract information for your application. This section must be no longer than 30 lines of text. The overall objective of this proposal is to accelerate the acquisition of structural information about membrane proteins by applying a structural genomics approach informed by the collective experience of a team of expert investigators. We have established the New York Consortium on Membrane Protein Structure (NYCOMPS) to work together toward this objective. As constituted for PSI-Biology, NYCOMPS will comprise ten Principal Investigators at five institutions. Our pipeline for structure determination will select targets through a bioinformatics analysis of known sequences, move on to recombinant DNA cloning, protein expression in bacteria or eukaryotic cells, and protein purification at moderately high throughput, and then proceed to determine structures by x-ray crystallography. Our Protein Production Facility at the New York Structural Biology Center (NYSBC) handles targets through purification at a mid-scale level; and successful candidates are distributed to participant laboratories for scale-up and crystallization. Minimal functional analysis of structures will be performed both by computations and through routine experimental biochemistry. Targets will be identified through nominations from the biological community, including adjunct NYCOMPS members, and from NYCOMPS biological themes, which concern elucidation of the membrane protein universe and structural studies on energy homeostasis and metabolic disorders. Technology development will be limited to direct improvements in pipeline efficiency. The project will be managed to optimize output and to integrate effectively with the PSI-Biology network and with other membrane protein structure efforts.