Campylobacter jejuni flagellar regulation and synthesis

空肠弯曲杆菌是许多动物胃肠道的共生细菌，尤其是 鸟。然而，当空肠弯曲菌感染人类的胃肠道时，这种细菌往往会引起一种 轻至重，血性腹泻综合征。空肠弯曲菌目前被认为是食源性肺炎的主要原因。 细菌性胃肠炎在世界范围内的人类。空肠弯曲杆菌的鞭毛已被鉴定为一种毒力 以及促进发病和共生的殖民因素。然而，知识 与其他细菌相比，空肠弯曲菌鞭毛基因的调控和生物合成是有限的。我们 发现空肠弯曲菌严格控制依赖sigma 54的鞭毛基因的转录 鞭毛的基本成分。这项提案的总体目标是确定如何 关键鞭毛蛋白和调节蛋白的功能融合以控制依赖于sigma 54的转录 鞭毛基因。拟议研究的中心假设是空肠弯曲菌协调需要FlhF， 鞭毛输出装置蛋白和flgs/flgR双组分转录调控系统 Sigma 54依赖于鞭毛运动所必需的基因。该项目的具体目标是：1) 评估FlhF在鞭毛基因调控和生物合成中的作用；2)确定鞭毛如何组装 影响依赖sigma 54的鞭毛基因转录的激活；以及3)研究可能的 调节鞭毛基因转录和生物合成的附加层，并决定这些鞭毛如何 调节机制可能会影响鸟类的共生关系。实现这些目标将有助于：1) 了解关键鞭毛蛋白之间的联系及其如何影响遗传机制 控制鞭毛基因调控和生物合成；2)增加对遗传调控的理解 空肠弯曲菌控制毒力和定植因子表达的途径。 空肠弯曲菌在其表面产生一种称为鞭毛的结构，这是 活动，促进人类腹泻病，以及动物的殖民。拟议的研究 活性将决定某些蛋白如何共同作用来控制鞭毛的产生。 构成鞭毛结构的蛋白质。

Campylobacter jejuni is a commensal bacterium of the gastrointestinal tracts of many animals, specifically birds. However, when C. jejuni infects the gastrointestinal tracts of humans, the bacterium often causes a mild to severe, bloody diarrheal syndrome. C. jejuni is currently recognized as a leading cause of food-borne bacterial gastroenteritis in humans world wide. The flagellum of C. jejuni has been identified as a virulence and colonization factor involved in promoting both pathogenesis and commensalism. However, knowledge regarding flagellar gene regulation and biosynthesis in C. jejuni is limited compared to other bacteria. We have found that C. jejuni tightly controls transcription of sigma 54-dependent flagellar genes encoding essential components of the flagellum. The overall objective of this proposal is to determine how the functions of key flagellar and regulatory proteins converge to control sigma 54-dependent transcription of flagellar genes. The central hypothesis of the proposed research is that C. jejuni coordinately requires FlhF, the flagellar export apparatus proteins, and the FlgS/FlgR two-component regulatory system for transcription of sigma 54-dependent genes essential for flagellar motility. The specific aims of this project are: 1) to assess the role of FlhF in flagellar gene regulation and biosynthesis; 2) to determine how flagellar assembly influences activation of transcription of sigma 54-dependent flagellar genes; and 3) to investigate possible additional layers of regulating flagellar gene transcription and biosynthesis and determine how these flagellar regulatory mechanisms may influence commensalism in birds. Accomplishment of these aims will aid in: 1) understanding connections between key flagellar proteins and how they influence genetic mechanisms to control flagellar gene regulation and biosynthesis; and 2) increase our understanding of genetic regulatory pathways used by C. jejuni for controlling expression of virulence and colonization factors. The bacterium Campylobacterjejuni produces a structure on its surface called a flagellum that is required for motility, promotion of diarrheal disease in humans, and colonization of animals. The proposed research activities will determine how certain proteins of C.jejunifunction together to control production of flagellar proteins that make up the flagellar structure.