Modeling of S.Aureus Transmission in Northern Manhattan

描述（由研究者提供）：此修订补充是对题为“使rpg能够利用NCRR中心和类似中心的项目”的通知no - od -09-058的回应。本次修订的目的是利用我们正在进行的R01的现有框架：“北曼哈顿的金黄色葡萄球菌传播”。总体目标是确定菌株ST398作为我们社区新出现的病原体的重要性，并确定其毒力的基础。我们的新目标如下。a. ST398在曼哈顿北部的宿主和传播方式是什么？基于集群的研究设计将用于确定曼哈顿北部ST398传播的基础。被ST398定植或感染的受试者将被识别、访谈和培养。所有接触者及其次要接触者将被确定并要求参与。将确定与该菌株的获得和传播有关的因素。将努力招收所有网络成员，包括最近在多米尼加共和国的联系人。根据过去ST398的动物来源历史，我们将研究社区中潜在的动物宿主，包括宠物店、兽医、屠夫和宠物美容师。确定菌株位置的空间制图也将用于确定潜在的传播模式或水库。b.哪些基因改变导致菌株ST398适应人与人之间的传播？我们将使用全基因组测序将北曼哈顿ST398分离株与与动物相关感染直接相关的分离株进行比较。我们将确定ST398基因组的独特区域，并可能解释这些克隆增加的传播性和毒力。将开发一种分子信标检测方法，以更容易地识别ST398。这将允许在大量金黄色葡萄球菌分离株中更有效和快速地筛选ST398。该研究将利用哥伦比亚大学欧文临床和转化研究中心的资源来帮助开展这项以社区为基础的调查。此次修订将提供两个新职位，并为全基因组测序公司提供额外的工作，以协助实现目标2。

DESCRIPTION (provided by investigator): This revised supplement is responsive to the notice NOT-OD-09-058 entitled: "Enabling RPGs to Leverage NCRR Center and Center-like Programs". The aims of this revision are designed to utilize the existing framework of our ongoing R01 entitled: "S. aureus transmission in Northern Manhattan." The overall goal is to define the importance of strain ST398 as an emerging pathogen in our community and to determine the basis for its virulence. Our new aims are listed below. a. What are the reservoirs and modes of transmission of ST398 in Northern Manhattan? A cluster-based study design will be used to define the basis for ST398 transmission in Northern Manhattan. Subjects either colonized or infected with ST398 will be identified, interviewed, cultured. All contacts and their secondary contacts will be identified and asked to participate. Factors associated with acquisition and transmission of this strain will be determined. Efforts will be made to enroll all network members including recent contacts in the Dominican Republic. In light of the past history of an animal origin for ST398 we will examine potential animal reservoirs in the community including pet stores, veterinarians, butchers and pet groomers. Spatial mapping of the location the strains were identified will also be used to identify potential patterns of or reservoirs for transmission. b. What genetic alterations are responsible for the adaptation of strain ST398 to person-to-person transmission? We will use total genome sequencing to compare the Northern Manhattan ST398 isolates with those isolates that have been directly associated with animal related infections. We will identify regions of the ST398 genome that are unique and might explain the increased transmissibility and virulence of these clones. A molecular beacon assay will be developed to more easily identify ST398. This will allow more efficient and rapid screening for ST398 in large collections of S. aureus isolates. The study will utilize the resources of the Columbia University Irving Center for Clinical and Translational Research to help carry out this community-based investigation. The revision will provide two new positions as well as provide additional work for the total genome sequencing company assisting with aim 2. PUBLIC HEALTH RELEVANCE: The goal of this revised supplement is to define the importance of S. aureus strain ST398, a strain originally associated with infections among animal workers now prevalent in Northern Manhattan, as an emerging pathogen in our community and to determine the basis for its virulence.