Mouse and Transplantation Core

鼠标和移植核心将全面负责培训 同种异体皮肤移植及移植物存活监测。它还将负责 病毒处理的实验动物在BSL2/3级生物遏制中的维护、治疗和外科操作。所有计划项目实验将使用VAF设施或旨在保持最高清洁度的有限准入生物遏制设施中饲养的VAF动物。对严格的有限获取的生物遏制的重视将防止意外传染病病原体的交叉污染和结果腐败。 动物核心股的目标和职责如下： 通过管理或培训人员正确使用试剂，包括病毒、抗CDI 54单抗、其他单抗、细胞、TLR激动剂和其他将在计划过程中开发的特定试剂，从而为VAF中的动物和生物遏制中的动物提供管理或培训。 通过制备造血学“同源嵌合体”，一种对移植成功至关重要的动物模型系统 项目1-3。 ？从生物围栏中的动物采购细胞、活检和身体标本，以进行组织学和流式细胞术分析。 ？动物核心人员将为所有项目执行或协助执行皮肤移植。 对实验动物进行血清学测试，以证明已发生感染，并且没有交叉污染。 简而言之，老鼠和移植核心将为每个项目提供必要的援助和人员培训，管理和执行在指定的VAF设施和在严格控制的感染条件下进行的体内研究。

The Mouse and Transplantation Core will have overall responsibilities for training all Projects in the transplantation of skin allografts and monitoring for graft survival. It will also have responsibility for maintenance, treatment, and surgical manipulation of virus treated experimental animals in BSL2/3 level biocontainment. All Program Project experiments will utilize VAF animals housed in a VAF facility or in a limited access biocontainment facility designed to maintain the highest degree of cleanliness. The emphasis on strict limited-access biocontainment will preclude cross-contamination and corruption of outcomes by unintended infectious agents. The objectives and responsibilities ofthe Animal Core Unit are as follows: By administering or training personnel in proper administration of reagents, including viruses, anti-CDI 54 mAb, other monoclonal antibodies, cells, TLR agonists, and other specific agents to be developed in the course ofthe Program to animals in VAF and in biocontainment. By preparing hematopoietic "synchimeras," an animal model system that is crucial for the success of Projects 1-3. ¿ Procurement of cells, biopsy and autopsy specimens for histological and flow cytometric analysis from animals in biocontainment. ¿ Animal Core personnel will perform or assist with performing skin grafts for all Projects. ¿ Serological testing of experimental animals to document that infection has occurred and that there is no cross-contamination. In brief, the Mouse and Transplantation Core will provide essential assistance and personnel training to each Project with the management and execution of in vivo studies conducted in defined VAF facilities and in strictly controlled infectious conditions in biocontainment.