Magneto-Nano Protein Chip and Multiplex Sorter for Monitoring Tumor Markers

我们正在开发一种磁纳传感器蛋白质芯片和一种基于磁力传感器的多重磁力分选器 纳米颗粒可以将离散的生物分子结合事件快速转化为电信号。 这些纳米技术可以在不到一小时的时间内检测到单个分子级别的目标分子。 这样的灵敏度和检测速度在目前的技术中是无法获得或不切实际的。两大主打 该项目的组件，磁性纳米传感器芯片和基于纳米颗粒的磁性分选器，可以 独立运行或联合运行。这种超纳米传感器芯片将识别和定量蛋白质。 小鼠和人血清样本中的肿瘤标志物和相关蛋白质谱史无前例 敏感性和特异性。这些磁纳米传感器通过表现出显著的电阻变化来发挥作用 它们仅由外部磁场感应，因此对溶液条件不敏感 作为缓冲液、pH或离子强度。生物传感是通过亲和标记两个传感器来完成的 表面和磁性纳米颗粒同时附着在特定靶向的不同结构域上 生物分子。然后，磁纳米传感器通过 磁性纳米颗粒所产生的磁场。对于足够小的传感器和适当的 磁性纳米颗粒标签，由于单个亲和力结合，特定的分子可以被检测为简单的 改变传感器的电阻，使昂贵的激励源或远程传感器 必填项。磁性分选器将快速分离生物分子。基于可调谐磁体 通过使它们以不同的速度偏转来约束它们的磁性纳米颗粒的性质 在给定的磁场和梯度下。特别是，我们将扩展技术以捕获和 从小鼠和人血清样本中鉴定循环肿瘤细胞，并随后分析 用于监测癌症治疗的细胞裂解物。

We are developing a magneto-nano sensor protein chip and a multiplex magnetic sorter based on magnetic nanoparticles that allow rapid conversion of discrete biomolecule binding events into electrical signals. These nanotechnologies can detect target molecules down to the single molecule level in less than an hour. Such sensitivity and detection speed are unavailable or impractical with current technologies. The two main components of this project, a magneto-nano sensor chip and a nanoparticle-based magnetic sorter, can operate independently or in conjunction. The maqneto-nano sensor chip will recognize and quantitate protein tumor markers and relevant protein profiles in mouse and human serum samples with unprecedented sensitivity and specificity. These magneto-nano sensors function by exhibiting significant resistance changes which are induced solely by external magnetic fields and are therefore insensitive to solution conditions such as buffers, pH or ionic strength. Biological sensing is accomplished by affinity labeling both the sensor surface and magnetic nanoparticles to simultaneously attach to distinct domains of specifically targeted biological molecules. The magneto-nano sensor then detects the attachment of the biomolecules through the magnetic field induced by the magnetic nanoparticles. For sufficiently small sensors and appropriate magnetic nanoparticle tags, affinity bonding due to a single, specific molecule can be detected as a simple change in the sensor electrical resistance so that expensive excitation sources or remote sensors are not required. The magnetic sorter will rapidly segregate biomolecules. based upon the tunable magnetic properties of the magnetic nanoparticles which bind them, by causing them to deflect at different speeds under a given magnetic field and gradient. In particular, we will be extending technologies to capture and characterize circulating tumor cells from mouse and human serum samples and subsequently analyze the cell lysate for monitoring cancer therapy.