Analysis Of Mechanisms Of Testicular Toxicity Using DNA Microarray Technology

睾丸中基因表达模式对镉和砷（已知的男性生殖毒物）的反应的变化被用于：1)确定对毒物的初级和次级基因反应，2)确定睾丸中毒物的初始靶细胞，以及3)使用基因表达变化的时间模式来确定毒性机制。

Changes in patterns of gene expression in the testis in response to cadmium and arsenic, known male reproductive toxicants are being used to: 1) identify primary and secondary gene responses to the toxicants, 2) to identify the initial target cell of the toxicants in the testis, and 3) to use the temporal pattern of changes in gene expression to define the mechanisms of toxicity. A mouse testis microarray has been developed and is being used in these studies. It also will be useful for studies of effects of gene knockouts, endocrine manipulations, and other treatments. Eight cDNA libraries constructed with mRNA from mouse testis and germ cells isolated at different stages of development were subjected to single-pass sequencing from the 5' end to identify clones to be used for this purpose. Over 28,000 clones were sequenced and over 22,000 ESTs were deposited in public databases. A mouse testis 22K microarray has been constructed using this sequence information and other information public databases. A comparison of the information gained from using the testis microarray and a whole mouse genome microarray has determined that a substantially larger number of genes on the testis microarray are seen to undergo changes in expression following cadmium treatment than are seen on the whole mouse genome microarray.