Cell Culture Resource Core

核心B是原代内皮细胞培养和腺病毒的中心资源，可用于每个 四个项目。这一核心设施将为以下方面提供服务：1)初级 人肺微血管内皮细胞；2)原代肺的分离、鉴定和培养 小鼠来源的微血管内皮细胞，包括不同基因小鼠模型的微血管内皮细胞和3) 重组腺病毒的扩增和纯化，用于以下项目的实验 在下面的核心描述中被取消。人和小鼠肺内皮细胞培养对 完成所有四个项目中提出的研究。具体地说，核心B对于提供 从不同的遗传小鼠模型中分离出内皮细胞；例如，fikGPF*‘“转基因和Cav-1，eNOS， 项目1的AKT-1、Src、NOX2和PAR-1基因敲除小鼠；eNOS、iNOS、NADPH氧化酶(p47Phox和NOX2) 和项目2的PAR-1基因敲除小鼠；TRPC6、TRPC1和TRPC4、Src、PKCα、PAR-1和EC-MLCK 项目3的基因敲除小鼠和项目4的pi 10γ、Src、Akt1、Cav-1、ICAM-1小鼠。核心B将 为所有四个项目提供重组腺病毒的扩增和纯化。这个组织的活动 核心是方案的成功和成功完成四个项目中所有目标的关键。

Core B is a central resource for primary endothelial cell cultures and adenoviruses available for each of the four projects. This core facility will provide services for the following: 1) characterization and culture of primary human lung microvascular endothelial cells; 2) isolation, characterization and culture of primary lung microvascular endothelial cells of mouse origin including those of different genetic mouse models and 3) amplification and purification of recombinant adenoviruses for the experiments proposed in the Projects as outiined in the Core description below. Human and mouse lung endothelial cell cultures are pivotal for the accomplishment of studies proposed in all four Projects. Specifically, Core B is critical for providing endothelial cells isolated from different genetic mouse models; e.g.,fiklGPF*'"transgenic and Cav-1, eNOS, AKT-1, Src, Nox2 and PAR-1 knockout mice for Project 1; eNOS, iNOS, NADPH oxidase {p47phox and Nox2) and PAR-1 knockout mice for Project 2; Trpc6, TRPC1 and TRPC4, Src, PKCα, PAR-1 and EC-MLCK knockout mice for Project 3 and pi 10γ, Src, Aktl, Cav-1, ICAM-1 mice for Project 4. In addition. Core B will provide amplification and purification of recombinant adenoviruses for all four Projects. The activities of this Core are essential for the success of the Program and successful completion of all aims in the four projects.