Structure-Function Studies Of DNA Replication Fidelity

今年，我们的团队经常与其他人合作，发表了六项与DNA复制保真度有关的初步研究。我们发现DNA聚合酶Delta在复制端粒DNA序列时停滞。与当前文献的预期相比，令人惊讶的是，这种停滞与DNA中G-四链的形成无关。在与R.Scott Williams小组的合作中，我们证明了aprataxin可以解决腺苷化的RNA-DNA连接，以保持基因组的完整性。我们与L.C.Pedersen合作，对B家族DNA复制酶绕过核苷酸的结构和功能进行了分析。我们还继续与Pedersen博士合作，并增加了与M.Garcia-Diaz的新合作，对参与修复物理和化学环境应激造成的DNA损伤的X家族DNA聚合酶lambda和u进行了三项新的结构-功能研究。此外，我们还发表了一篇方法学文章、两篇综述文章和一篇文章，重点介绍了DNA复制酶学方面的一项重大突破。

This year our group, often in collaboration with others, published six primary studies related to DNA replication fidelity. We showed that DNA polymerase delta stalls when replicating telomeric DNA sequences. Surprisingly compared to expectations from the current literature, this stalling occurs independent of formation of G-quadruplexes in DNA. In collaboration with R. Scott Williams group, we showed that aprataxin resolves adenylated RNA-DNA junctions to maintain genome integrity. In collaboration with L.C. Pedersen, we performed a structure-function analysis of ribonucleotide bypass by B family DNA replicases. We also continued to collaborate with Dr. Pedersen, and added a new collaboration with M. Garcia-Diaz, to perform three novel structure-function studies of Family X DNA polymerases lambda and mu that participate in repairing DNA damage resulting from physical and chemical environmental stressors. In addition, we published a methodology article, two review articles and an article highlighting a major breakthrough in DNA replication enzymology.