Urokinase-type plasminogen activator in the ischemic brain

 描述(申请人提供)：尿激酶型纤溶酶原激活剂(UPA)是一种丝氨酸蛋白酶，可与尿激酶型纤溶酶原激活剂受体(UPAR)结合。虽然最初认为uPA-uPAR结合的主要作用是催化纤溶酶原在细胞表面转化为纤溶酶，但很快发现uPA-uPAR还激活细胞信号通路，以响应细胞外基质(ECM)组成的变化，导致组织重塑、伤口愈合以及细胞黏附和迁移。UPA和uPAR在成人大脑皮质神经元中表达。在这项应用中，我们将测试这一假设，即在坏死核周围区域，uPA与uPAR的结合促进了缺血性中风后树突棘的恢复和功能改善。

 DESCRIPTION (provided by applicant): Urokinase-type plasminogen activator (uPA) is a serine proteinase that binds to the urokinase plasminogen activator receptor (uPAR). Although it was initially believed that the main role of uPA-uPAR binding was to catalyze the conversion of plasminogen into plasmin on the cell surface, it was soon discovered that uPAR also activates cell signaling pathways in response to changes in the composition of the extracellular matrix (ECM) leading to tissue remodeling, wound healing, and cell adhesion and migration. uPA and uPAR are expressed in cerebral cortical neurons in the adult brain. In this application we will test the hypothesis that binding of uPA to uPAR in the area surrounding the necrotic core promotes dendritic spine recovery and functional improvement after an ischemic stroke.