Core C RNA Sequencing and Bioinformatics

RNA测序已迅速发展成为转录组学的革命性工具。目前，它是一个 用于评估转录本丰度和变体的最敏感和最全面的工具 在各种生物样本中。在这项应用中，RNA-seq Core将在以下方面发挥至关重要的作用： 对所有项目获得的样本进行转录组学和生物信息学分析。 RNA-seq/Bioinformatics Core的具体目标是： 1.为每个项目提供RNA测序（RNA-seq）服务。这包括 对多聚A+ RNA进行测序以定量组织中的多聚腺苷酸化转录物。 2.对RNA-seq数据进行生物信息学分析。这将包括相对RNA 丰度、剪接变异和非人ORF的存在。 核心功能：核心功能将以一般术语描述，然后与 以上列出的具体目标。

RNA sequencing has quickly evolved to be a revolutionary tool for transcriptomics. Currently, it is one of the most sensitive and comprehensive tools for assessment of transcript abundance and variants in various biological samples. In this application, the RNA-seq Core will perform a vital role in performing transcriptomics and bioinformatic analyses of samples obtained from all projects. The specific aims of the RNA-seq/Bioinformatics Core are to: 1. Perform RNA sequencing (RNA-seq) services for each of the projects. This includes sequencing of poly A+ RNA to quantify polyadenylated transcripts in tissues. 2. Perform bioinformatic analysis of the RNA-seq data. This would include relative RNA abundance, splice variation, and presence of non-human ORFs. Core Function: The core functions will be described in general terms and then in relation to the specific objectives listed above.