Flow Cytometry Laboratory (Scientific Core)

虽然流式细胞术实验室是位于巴尔的摩的NIA内部研究计划内的实验室的科学使命的核心，但几个调查人员、实验室小组或项目占据了2009财年资源的重要部分。免疫学实验室(Li；Taub，Weng，Sen)的使用从60%-70%的分类能力到50%-70%的非分类(分析)能力，重点放在免疫状态、免疫调节、免疫抑制及其与发育和衰老的关系的人和动物(鼠)模型上。其他得到资助的项目包括心血管科学实验室(LCS：Boheler，ES细胞与心肌细胞分化；肖，报告基因表达)，遗传学实验室(LG：KO，ES发育模式)，神经科学实验室(LNS：Mattson，神经干细胞与神经发生中的报告基因表达)，临床调查实验室(Bernier)，分子老年学实验室(LMG：Gearhart，抗体多样性和体细胞超突变)，以及细胞和分子生物学实验室(LCMB；多参数细胞周期分析)。虽然实验室并不要求我们为其生成数据的所有出版物都是共同作者，但除了下面的参考书目外，以下清单还记录了工作组的出版物，其中包括本报告所述期间我们的核心人员所作的贡献。 1)Schurman SH，Hedayati M，Wang Z，Singh DK，Speina E，Zhang Y，Becker K，Macris M，Sung P，Wilson DM Third，Croteau DL，Bohr VA。(2009)。RECQL4在调节碱基切除修复能力中的直接和间接作用。嗯，摩尔·吉内特。18(18)：3470-83。 2)于强，夏尔玛A，吴希，穆海刚，侯赛因MZ，Salay TM，利兹柯，杜H，吴斌，沃特曼ML，朱Z，Sen JM。(2009)。T细胞因子1通过诱导转录因子GATA-3和抑制干扰素-γ来启动T辅助细胞2型命运。纳特免疫。10(9)：992-9。 3)徐明，夏尔玛A，侯赛因MZ，Wiest DL，Sen JM。(2009)。前TCR诱导的β-连环蛋白在β-选择后胸腺细胞中的持续表达阻止T细胞的发育。j免疫。182(2)：759-65。 4)徐明、夏尔玛·A、威斯特·迪尔、森·JM。(2009)。Pre-TCR诱导的β-连环蛋白促进通过β-选择的遍历。182(2)：751-8。 5)Ghosh MC，Collins GD，Vandanmagsar B，Patel K，Brill M，Carter A，Lustig A，Becker KG，Wood WW Third，Emeche CD，法语AD，O‘Connell MP，Xu M，Weeraratna AT，Taub DD。(2009)。CXC趋化因子配体12介导的T细胞迁移需要Wnt5A信号的激活。血。114(7)：1366-73。 6)Lustig A，Carter A，Bertak D，Enika D，Vandanmagsar B，Wood W，Becker KG，Weeraratna AT，Taub DD。(2009)对小鼠胸腺细胞的转录组分析揭示了胸腺基因表达的年龄相关变化。INJ医学科学。6(1)：51-。 7)McNeill博士，Lam W，DeWeese TL，Chen YC，Wilson DM第3名。(2009)。APE1功能受损增强了细胞对临床相关烷化剂和抗代谢药物的敏感性。摩尔癌症研究7(6)：897-906。 8)Dixit VD，Yang H，Cooper-Jenkins A，Giri BB，Taub DD。(2009)。T细胞来源的Ghrelin的减少增强了促炎细胞因子的表达：与年龄相关的炎症增加的意义。血液113：5202-5205。 9)Muniandy PA，Thapa D，Thazhathveester AK，刘S，Seidman MM(2009年)。激光定位的G1期哺乳动物细胞DNA链间交联的修复。《生物化学学报》284：27908-27917。 10)Ghosh MC，BatalD，Collins G，Carter A，Indig F，Biragyn A，Taub DD。(2009)地塞米松通过激活Src激酶Lck增强CXCR4介导的静息人类T细胞信号转导。血。113：575-584。 11)Aggarwal M，Sommers JA，Morris C，Brosch RM Jr.(2010)用EXO1描述了WRN解旋酶在复制应激反应中的功能。DNA修复9：765-776。 12)刘静，马琼达A，刘静，汤普森，谢德曼MM(2010)。单链寡核苷酸供体通过非同源末端连接在哺乳动物细胞中进行的序列转换。《生物化学学报》285：23198-27207。 13)阮氏，翁NP。(2010)在整个成年阶段，IL-21优先促进IL-15介导的人CD28+CD8记忆T细胞的动态平衡增殖。J白血球生物。87：43-49。

While the Flow Cytometry Laboratory functions as a core for the scientific mission of the laboratories located within the NIA Intramural Research Program in Baltimore, several investigators, laboratory groups, or projects accounts for a significant portion of FY2009 resources. Usage by the Laboratory of Immunology (LI; Taub, Weng, Sen) varies between 60-70% of sorting and 50-70% of non-sorting (analytical) capacities, focusing on human and animal (mouse) models of immune status, immunoregulation, immunosuppression, and their relation to development and aging. Other supported projects involve those from the Laboratory of Cardiovascular Sciences (LCS: Boheler, ES cells and cardiomyocte differentiation; Xiao, reporter gene expression), Laboratory of Genetics (LG: Ko, patterns of ES development), Laboratory of Neuroscience (LNS: Mattson, reporter gene expression in neural stem cells and neurogenesis), Laboratory of Clinical Investigation (Bernier), Laboratory of Molecular Gerontology (LMG: Gearhart, antibody diversity and somatic hypermuations), and Laboratory of Cellular and Molecular Biology (LCMB; multiparameter cell cycle analysis). While the lab does not require co authorship on all publications for which we generate data, the following list, in addition to the bibliography below, documents publications from the IRP that include contributions from our core for this reporting period. 1) Schurman SH, Hedayati M, Wang Z, Singh DK, Speina E, Zhang Y, Becker K, Macris M, Sung P, Wilson DM 3rd, Croteau DL, Bohr VA. (2009). Direct and indirect roles of RECQL4 in modulating base excision repair capacity. Hum Mol Genet. 18(18):3470-83. 2) Yu Q, Sharma A, Oh SY, Moon HG, Hossain MZ, Salay TM, Leeds KE, Du H, Wu B, Waterman ML, Zhu Z, Sen JM. (2009). T cell factor 1 initiates the T helper type 2 fate by inducing the transcription factor GATA-3 and repressing interferon-gamma. Nat Immunol. 10(9):992-9. 3) Xu M, Sharma A, Hossain MZ, Wiest DL, Sen JM. (2009). Sustained expression of pre-TCR induced beta-catenin in post-beta-selection thymocytes blocks T cell development. J Immunol. 182(2):759-65. 4) Xu M, Sharma A, Wiest DL, Sen JM. (2009). Pre-TCR-induced beta-catenin facilitates traversal through beta-selection.J Immunol. 182(2):751-8. 5) Ghosh MC, Collins GD, Vandanmagsar B, Patel K, Brill M, Carter A, Lustig A, Becker KG, Wood WW 3rd, Emeche CD, French AD, O'Connell MP, Xu M, Weeraratna AT, Taub DD. (2009). Activation of Wnt5A signaling is required for CXC chemokine ligand 12-mediated T-cell migration. Blood. 114(7):1366-73. 6) Lustig A, Carter A, Bertak D, Enika D, Vandanmagsar B, Wood W, Becker KG, Weeraratna AT, Taub DD. (2009) Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression. Int J Med Sci. 6(1):51-64. 7) McNeill DR, Lam W, DeWeese TL, Cheng YC, Wilson DM 3rd. (2009). Impairment of APE1 function enhances cellular sensitivity to clinically relevant alkylators and antimetabolites. Mol Cancer Res. 7(6):897-906. 8) Dixit VD, Yang H, Cooper-Jenkins A, Giri BB, and Taub DD. (2009). Reduction of T-cell derived ghrelin enhances proinflammatory cytokine expression: implications for age-associated increases in inflammation. Blood 113:5202-5205. 9) Muniandy PA, Thapa D, Thazhathveetil AK, Liu S, Seidman MM. (2009). Repair of Laser-localized DNA Interstrand Cross-links in G1 Phase mammalian Cells. Journal of Biological Chemistry 284:27908-27917. 10) Ghosh MC, Baatar D, Collins G, Carter A, Indig F, Biragyn A, Taub DD. (2009) Dexamethasone augments CXCR4-mediated signaling in resting human T cells via the activation of the Src kinase Lck. Blood. 113:575-584. 11) Aggarwal M, Sommers JA, Morris C, Brosch RM Jr. (2010) Delineation of WRN helicase function with EXO1 in the replicational stress response. DNA Repair 9:765-776. 12) Liu J, Majumdar A,Liu J, Thompson LH, Seidman MM. (2010) . Sequence Conversion by Single Strand Oligonucleotide Donor via Non-homologoud End Joinng in Mammalian Cells. Journal of Biological Chemistry 285:23198-27207. 13) Nguyen H, Weng NP. (2010) IL-21 preferentially enhances IL-15-mediated homeostatic proliferation of human CD28+ CD8 memory T cells throughout the adult age span. J Leukocyte Biol. 87:43-49.