TAS::75 0849::TAS DEVELOPMENT A PROPRIETARY TIP-ENHANCED RAMAN SPECTROSCOPY (TER

基因DNA的突变是导致癌症的一个主要因素。在单个分子的DNA序列中定位这些变化是困难的，因为它们不能通过传统的测序技术进行分析。该项目将展示固定在新型底物上的单链DNA和RNA的测序能力，并展示一种提高通量的新方法。对中序列病变和碱基修饰（如8-氧鸟苷、碱基甲基化和其他与癌症相关的核苷酸修饰）的敏感性也将被证明。

Mutation of genetic DNA is a major factor causing cancer. Locating these changes in the DNA sequence of single molecules is difficult because they cannot be assayed by conventional sequencing techniques. This project will demonstrate sequencing capabilities for single stranded DNA and RNA immobilized on a novel substrate, and demonstrate a novel method for increasing throughput. Sensitivity to mid-sequence lesions and base modifications such as 8-oxoguanosine, base methylation, and other cancer-relevant nucleotide modifications will also be demonstrated.