Ionic currents and spiking in cerebellar nuclear neurons

描述（由申请人提供）：小脑核的神经元是自发活性神经元，其整合来自苔藓纤维的兴奋性输入和来自浦肯野神经元的抑制性输入以产生小脑的输出。在真实的时间里，小脑输出纠正错误，促进学习和协调运动，提出了抑制，兴奋和内在放电如何相互作用来调节小脑放电模式的问题。 核细胞目前的建议是针对测试的想法，即核细胞的反应浦肯野介导的抑制根据小脑在任何时候产生的校正或调节而变化。特别是，抑制性同步的程度可能决定小脑输出的任务依赖的方式。实验将在体外和体内进行，在正常和突变小鼠和斑马鱼。将使用电压钳、电流钳和细胞外电生理记录来测试练习运动行为期间和错误期间浦肯野和核细胞活性的相关性变化、兴奋性与抑制性输入的相互作用以及学习期间浦肯野同步性和核细胞输出的变化。

DESCRIPTION (provided by applicant): Neurons of the cerebellar nuclei are spontaneously active neurons that integrate excitatory input from mossy fibers and inhibitory input from Purkinje neurons to generate the output of the cerebellum. In real time, cerebellar output corrects errors and facilitates learned, coordinated movements, raising the question of how inhibition, excitation, and intrinsic firing interact to regulate the firing patterns of cerebellar nuclear cells. The present proposal is directed toward testing the idea that nuclear cells' response to Purkinje-mediated inhibition varies according to the correction or regulation produced by the cerebellum at any time. In particular, the degree of inhibitory synchrony may dictate cerebellar output in a task-dependent manner. Experiments will be performed both in vitro and in vivo, in normal and mutant mice and in zebrafish. Voltage-clamp, current-clamp, and extracellular electrophysiological recordings will be used to test the changes in correlation of Purkinje and nuclear cell activity during practiced motor behaviors and during errors, the interaction of excitatory with inhibitory inputs, and the changes in Purkinje synchrony and nuclear cell output during learning.