Mutant Mouse Core

突变鼠核心将集中力量，提供必要的专业知识，以维持老鼠的库存。 通过基因靶向和/或转基因插入产生的各种突变， investigators.需要具有特定靶向突变和/或转基因的小鼠的研究者将能够 从核心获得所需数量的适当基因型。因此，核心负责 建立者和繁殖动物的常规护理，产生实验动物所需的饲养， 它们的断奶、用于鉴定的耳标的应用以及获得相关基因的基因型。的 如果市场上没有合适的质控品，则Core还负责提供。 将与每名研究者协调开展育种计划，以获得适当的数量 所需基因型的动物。我们将在PPG更新中向研究者提供以下信息 基因敲除小鼠;血管紧张素1受体1a型“floxed”（ATIa-floxed）-/-，血管紧张素II受体2型 (AT2)-/Y，内皮型一氧化氮合酶（Nos 3或eNOS）-/-，上皮细胞的条件性敲除 钠通道（ENaC）在皮质集合管（CCD）单独和CCD加连接 微管（CNT）和心肌细胞特异性敲除前列腺素EP 4受体（CS-EP 4）-/-。我们 将提供以下转基因小鼠：NF-κ B/荧光素酶（NF-κ B-LUC）Tg，和鸡卵清蛋白特异性 a和p T细胞受体（OT II）Tg小鼠。此外，核心将提供小鼠，产生的同源 重组，其作为Liddle综合征的小鼠模型在PENaC基因中具有功能获得性。在 此外，中心将提供以下品系，根据需要直接购买作为实验动物， PPG研究者; Ragl -/-、p47-/-、NZBWF 1/J和NZW/LacJ。 相关性（参见说明）： PPG的突变小鼠核心，核心C将提供转基因小鼠，以支持 项目一至四的调查，如这些章节所述。提供这些核心服务 这将使研究人员能够专注于他们的实验，而不必担心他们自己。 小鼠的日常饲养和基因分型。

The Mutant Mouse Core will centralize effort and provide the expertise necessary to maintain stocks of mice with various mutations generated by gene targeting and/or transgene insertion for use by participating investigators. Investigators requiring mice with specific targeted mutations and/or transgenes will be able to obtain the appropriate genotypes in requisite numbers from the Core. The Core is thus responsible for the routine care of founder and breeding animals, the husbandry required to generate the experimental animals, their weaning, application of ear tags for identification, and obtaining genotypes for the relevant gene(s). The Core is also responsible for supplying suitable controls when they are not readily commercially available. Breeding programs will be carried out in coordination with each investigator to obtain the appropriate number of animals of the required genotypes. We will provide the investigators in the PPG renewal the following gene knockout mice; Angiotensin 1 receptor type l a "floxed" (ATIa-floxed) -/-, angiotensin II receptor type 2 (AT2) -/Y, endothelial nitric oxide synthase (Nos3 or eNOS) -/-, conditional knockouts of the a epithelial sodium channel (ENaC) in both the cortical collecting duct (CCD) alone and the CCD plus the connecting tubule (CNT), and cardiac myocyte specific knockout of the prostaglandin EP4 receptor (CS-EP4) -/-. We will provide the following transgenic mice: NF-KB/luciferase (NF-KB-LUC) Tg, and chicken ovalbumin specific a and p T-cell receptor (OT II) Tg mice. In addition the Core will provide mice, generated by homologous recombination, which have a gain-of-function in the PENaC gene as a mouse model of Liddle's syndrome. In addition the Core will provide the following strains, directly purchased as needed as experimental animals to PPG investigators; Ragl -/-, p47-/-, NZBWF1/J, and NZW/LacJ. RELEVANCE (See instructions): The Mutant Mouse Core of the PPG, Core C will provide genetically modified mice to support the investigations to be conducted in Projects l-IV, as described in those sections. Providing these core services will allow the investigators to concentrate on their experiments without having to concern themselves with the day-to-day husbandry and genotyping of mice.