Core B -Comprehensive Mouse Physiology and Phenotyping

工作总结：核心B -综合小鼠生理学和表型分析 全面的小鼠生理学和小鼠表型分析，包括压力超负荷手术模型， 引起心脏肥大和心力衰竭。PI的实验室在广泛的领域拥有相当的专业知识 小鼠表型分析的方法，并将提供核心服务的PPG的成员。的PI 他还参与了许多心力衰竭的遗传和外科模型的开发。为 例如，压力超负荷性心脏肥大的显微外科手术和血流动力学评价 由PI开发了通过横向主动脉缩窄诱导，现在被认为是常规方法 在世界各地的许多实验室中进行。经胸2D引导M型超声心动图，血流动力学 通过小鼠的压力-容积环和应力应变分析的收缩功能是标准程序， 其中许多是在PI的实验室开发的。游泳和轮跑的运动训练， 小鼠是目前使用的方法。

SUMMARY OF WORK: Core B – Comprehensive Mouse Physiology and Phenotyping Comprehensive mouse physiology and mouse phenotyping including surgical models of pressure overload to induce cardiac hypertrophy and heart failure. The PI's laboratory has considerable expertise in a wide-range of methodologies for the phenotyping of mice and will provide Core service to members of the PPG. The PI has also been involved in the development of numerous genetic and surgical models of heart failure. For example, microsurgical procedures and hemodynamic evaluation of pressure overload cardiac hypertrophy induced through transverse aortic constriction was developed by the PI, and is now considered a routine procedure in many labs around the world. Transthoracic 2D guided M-mode echocardiography, hemodynamic and contractile function by pressure-volume loops and stress strain analyses in mice are standard procedures, many which were developed in the PI's laboratory. Exercise training by both swimming and wheel running for mice are methods currently being used.