Chemical Synthesis and High Throughput Analytics Shared Resource

翻译课题组 CORE 010-化学合成和高通量分析共享资源 项目摘要/摘要 范德比尔特化学合成和高通量分析共享资源(CSHTASR)提供 范德比尔特-英格拉姆癌症中心(VICC)研究人员的最先进能力，以利用 化学和全基因组siRNA支持需要应用化学工具来回答的研究 生物问题。这一共享资源支持的项目分为以下几类：1)医药 化学，包括点击到铅和单个化学探针的合成，用于临床前或假说驱动的 体外研究；2)用于体内动物研究的大规模合成(10-100克)；3)同位素合成 标记化合物；4)亲和和荧光探针；5)修饰核苷酸和多肽的合成；以及 6)高通量分析仪器，可为多种实验设计提供读数范围 从生化到基于图像的表型分析；7)筛选高达75,000个全基因组siRNA文库 SiRNAs；8)非常多样化的化学库的低通量、中通量和高通量的化学筛选。这个 CSHTASR通过结合仪器技术和人员知识来提供这些服务。 机构和美国国立卫生研究院的资金已经投入，以获得有效实施 和高效的CSHTA服务。此外，CSHTASR的小分子和siRNA文库也很有价值 允许对正在研究的生物现象进行审问的物质资源，提供了对 化学探针优化的目标/途径识别和起点。CSHTASR采用了 训练有素的领导和支持人员，在先进的化学技术和 HTS方法论。协商方法用于通过共享资源进行的项目；这 是一种必要且有益的模式，允许VICC调查员和 共享资源的工作人员为每个人的需求提供最佳方法。

TRANSLATIONAL RESEARCH GROUP CORE 010 – CHEMICAL SYNTHESIS AND HIGH-THROUGHPUT ANALYTICS SHARED RESOURCE PROJECT SUMMARY/ABSTRACT The Vanderbilt Chemical Synthesis and High-throughput Analytics Shared Resource (CSHTASR) provides state-of-the-art capabilities for Vanderbilt-Ingram Cancer Center (VICC) researchers to harness the power of chemistry and whole genome siRNA to support research requiring the application of chemical tools to answer biological questions. Projects supported by this shared resource fall into the categories of: 1) medicinal chemistry, including hit-to-lead and individual chemical probe synthesis for preclinical or hypothesis-driven in vitro studies; 2) large-scale synthesis (10-100 grams) for in vivo animal studies; 3) synthesis of isotopically labeled compounds; 4) affinity and fluorescent probes; 5) synthesis of modified nucleotides and peptides; and 6) high-throughput analytics instrumentation that provide readouts for multiple experimental designs ranging from biochemical to image-based phenotypic assays; 7) whole genome siRNA library screens of up to 75,000 siRNAs; 8) low-, medium- and high-throughput chemical screens of very diverse chemical libraries. The CSHTASR provides these services through a combination of instrument technology and personnel knowledge. Institutional and NIH funds have been invested to acquire leading-edge instrumentation required for effective and efficient CSHTA services. In addition, the CSHTASR’s small molecule and siRNA libraries are valuable material resources that allow interrogation of the biological phenomena being studied, providing insight into target/pathway identification and starting points for chemical probe optimization. The CSHTASR employs a highly trained leadership and support staff with extensive experience in advanced chemistry techniques and HTS methodologies. A consultative approach is used for projects that come through the shared resource; this is a necessary and beneficial model that allows transfer of knowledge between VICC investigator and the shared resource staff to provide the optimal approach to each individual need.