Loyola Project

新出现的证据表明，膀胱并不像之前认为的那样是无菌的。这一证据还表明， 常驻细菌群落，女性尿路微生物群(FUM)，可能会影响多种尿路疾病， 包括急迫性尿失禁、尿路感染和可能的泌尿系疼痛综合征。这个 健康女性尿液细菌的来源可能为了解慢性前列腺炎的细菌病因提供重要的见解 对泌尿系统疾病的贡献。随着我们对以下方面的了解的深入，我们预计会产生重大的临床影响 可量化、可预测、可预防和/或可修改的排烟失调。为了增进这方面的知识，我们 请求资金来测试FUM受邻近骨盆微生物群影响的总体假设(即， 阴道和会阴)，而且FUM的成分与泌尿症状和结局密切相关。 我们建议对已有的尿样、阴道和会阴拭子样本进行表征。 出自&gt；300位性格鲜明的成年女性。利用16S rRNA序列分析和创新的生物信息学 分析，我们将表征这些样本中的细菌群落，以调查它们之间的关系 FUM和相邻骨盆壁龛的微生物群(目标1)，以及这些微生物群和 临床表现(目标2)。这些信息将迅速与更广泛的泌尿科研究社区共享 通过专门的网络数据库(AIM 3)，促进对不同临床情况的进一步研究；以及 产生未来的假设。

Emerging evidence shows that the bladder is not sterile as previously thought. This evidence also shows that the resident bacterial community, the female urinary microbiome (FUM), may impact multiple urinary disorders, including urgency urinary incontinence, urinary tract infections and possibly urologic pain syndromes. The origins of urinary bacteria in healthy women may provide important insights into the bacterial cause of or contribution to urinary disorders. We anticipate major clinical implications as we advance knowledge concerning FUM dysbioses that can be quantified, predicted, prevented and/or modified. To advance that knowledge, we request funds to test the overarching hypothesis that the FUM is influenced by adjacent pelvic microbiomes (i.e., vaginal and perineal) and that FUM compositions are strongly associated with urinary symptoms and outcomes. We propose to characterize a pre-existing repository of urine samples and vaginal and perineal swabs obtained from >300 well-characterized adult women. Using 16S rRNA sequence analysis and innovative bioinformatics analysis, we will characterize the bacterial communities in these samples to investigate relationships between the FUM and the microbiomes of adjacent pelvic niches (Aim 1), and between each of these microbiomes and clinical findings (Aim 2). This information will be promptly shared with the wider urological research community via a dedicated web-based database (Aim 3), facilitating further study of diverse clinical conditions, and generating future hypotheses.