Genomics of Antibiotic Resistance in Bacterial Pathogens

新出现的抗生素耐药性使鲍曼不动杆菌感染的治疗成为可能， 肺炎克雷伯菌和其他肠杆菌科细菌的疗效越来越差。许多抗性基因都是 携带可移动的遗传元素，并可在品系和物种之间很容易地交换。这个项目 将使用全面的基因组分析来提高我们对抗药性遗传背景的理解 基因、它们的染色体背景和横向基因转移。耐药元件的性质 将使用历史上和正在进行的分离株收集来分析人群中的传播过程 来自几家医院。更多的研究旨在探索混合感染在遗传中的作用 微生物群中病原体与共生菌的交换和关系。范围和 鲍曼不动杆菌和肺炎克雷伯菌的DNA甲基化程度将用单分子REAL进行分析 时间序列和对基因表达的影响将被确定。结果应该是有价值的 分子检测技术的发展有助于临床诊断和选择合适的治疗方法 感染。

Emerging antibiotic resistance is making treatment of infections caused by Acinetobacter baumannii, Klebsiella pneumoniae and other Enterobacteriaceae increasingly ineffective. Many resistance genes are carried on mobile genetic elements and can readily be exchanged between strains and species. This project will use comprehensive genome analysis to improve our understanding of the genetic context of resistance genes, their chromosomal background, and lateral gene transfer. Properties of the drug resistance element transmission process in populations will be analyzed using historical and ongoing collections of isolates from several hospitals. Additional studies are designed to explore the role of mixed infections in genetic exchange and the relationship of pathogens to commensal bacteria in the microbiome. The scope and extent of DNA methylation in A. baumannii and K. pneumoniae will be analyzed using single molecule real time sequencing and the effect on gene expression will be determined. The results should be of value in development of molecular assays to facilitate diagnosis and selection of appropriate therapies for clinical infections.