Diode array UV- visible spectrophotometer

目前的建议是购买一个二极管阵列紫外可见分光光度计，以支持萨斯喀彻温大学药学和营养学院的制药、生物和环境研究方案。 这件设备将安装在核心设备设施，一个新的多用户设备室，位于学院的中心位置，并容纳设备，可供所有教师，博士后研究员，研究生，技术人员和暑期学生在药学和营养学院。该机构聘请了一名经验丰富的实验室管理人员来维护设备，并对所有用户进行设备的正确维护和使用培训。二极管阵列紫外-可见分光光度计提供紫外-可见光谱的瞬时测量，并有助于表征单个药物和/或代谢物。 与传统的紫外可见分光光度计相比，它具有快速扫描、出色的灵敏度、上级波长再现性以及出色的耐用性和可靠性。 二极管阵列仪器的一个重要用途是在酶的动力学研究。 由于五氯苯酚及其催化产物难以用单一频率进行测定，而且某些催化产物的稳定性不足以用于传统高效液相色谱法的研究，因此，所要求的仪器对于我们目前进行的北美主要环境污染物五氯苯酚生物降解过程中所涉及的催化酶的动力学研究尤其重要。此外，使用二极管阵列紫外可见分光光度计获得的数据可以外推用于传统的HPLC-UV（单波长）检测器，从而可以使用更简单的技术实现最大的灵敏度。

The current proposal is for the purchase of a diode array UV-visible spectrophotometer to support the pharmaceutical, biological and environmental research programs in the College of Pharmacy and Nutrition at the University of Saskatchewan.  This piece of equipment will be installed in the Core Equipment Facility, a new multi-user equipment room which is centrally located in the College and houses equipment which is available to all faculty, postdoctoral fellows, graduate students, technicians and summer students in the College of Pharmacy and Nutrition. This facility employs an experienced lab manager to maintain the equipment and train all users in the proper care and use of the equipment. A diode-array UV-visible spectrophotometer provides instantaneous measurement of the UV-visible spectrum and aids in characterizing individual drugs and/or metabolites.  Compared to a conventional UV-visible spectrophotometer, it offers fast scanning, excellent sensitivity, superior wavelength reproducibility, as well as  exceptional ruggedness and reliability.  An important usage of diode-array instruments is in the kinetic studies of enzymes.  The instrument requested is especially crucial for our current kinetic studies of the catalytic enzymes involved in the biodegradation of pentachlorophenol, a major environmental pollutant in North America, because pentachlorophenol and it's catalytic products are difficult to measure by a single frequency and some of the catalytic products are not stable enough for the traditional high-performance liquid chromatography studies. Furthermore, the data obtained using the diode array UV Visible spectrophotometer can be extrapolated for use on conventional HPLC-UV(single wavelength)detectors such that maximum sensitivity can be achieved using simpler technology.