The dynamics of plastid protein trafficking mechanisms and organelle biogenesis

植物是地球生命力的支柱，这种力量对周围环境和地球的福祉具有深远的影响。 植物参与维持生命的活动，如光合作用、淀粉和油的生产以及呼吸作用。 这些活动由动态过程控制，需要无数分子参与者的相互作用，并在植物细胞内的专门空间中进行。 叶绿体和线粒体是重要的“生命驱动”细胞器，为植物细胞捕获和产生能量。 这些能量产生过程是所有细胞活动的基础，通过提供能量和产物来驱动蛋白质网络，并通过提供控制决策事件的调节信号。 这些决定反过来又决定了工厂在不断变化的环境下如何工作。 因此，细胞事件是由细胞核蛋白质和细胞器之间的相互作用网络驱动的。一个关键的相互作用机制涉及蛋白质递送。 蛋白质递送本身的动态行为（例如，控制酶、调节剂和看门人的传递）将决定蛋白质网络如何在不同的环境中反应和协同工作，更重要的是，决定细胞器如何发展以适应不断变化的环境。 我们的研究重点是蛋白质递送系统如何工作，以及如何调节这一过程以帮助植物适应不断变化的环境。 该提案概述了新的实时策略，以确定蛋白质递送在植物发育，适应和细胞器生物发生中的作用。 这一努力将导致在基础知识和商业上有用的技术方面具有价值的发现。 这些进展将为评估化学品（药物或环境）的影响提供强大的工具，为健康研究提供疾病模型，并为温室气体管理提供战略。 更重要的是，通过拟议的5年NSERC计划的学生将提供一个多学科的环境，用于适用于他们职业生涯的发现研究的强化培训（至少3个博士，6个硕士和10个学士）。

Plants form the pillars of the Earth's life force, a force that has far-reaching implications for the surrounding environment and the well-being of the planet.  Plants take part in life-supporting activities such as photosynthesis, starch and oil production, and respiration.  These activities are governed by dynamic processes, requiring interaction of countless molecular participants and take place in specialized spaces inside plant cells.  Chloroplasts and mitochondria are important "life-driving" organelles, capturing and producing energy for the plant cell.  These energy-generating processes underlie the workings of all cellular events, by providing energy and products for driving the protein networks and by providing the regulatory signals governing decision-making events.  These decisions in turn determine how the plant works under changing environments.  Cellular events are thus driven by a web of interacting networks between proteins of the nucleus and the organelles. A key interactive mechanism involves protein delivery.  The dynamic act of protein delivery itself (e.g., the delivery of controlling enzymes, regulators, and gatekeepers) will determine how protein networks react and work together in response to different environments, and more importantly, how organelles develop for adaptation to the ever-changing environment.  Our research is focused on how protein delivery systems work and how this process is regulated to help plants adapt to the changing environment.  This proposal outlines new, real-time strategies to map out the role of protein delivery in the development of a plant, in adaptation, and in organelle biogenesis.  This endeavour will lead to discoveries bearing value in terms of fundamental knowledge and commercially useful technologies.  The advancements will provide powerful tools for assessing the impact of chemicals (pharmaceutical or environmental), provide disease models for health research, and strategies for greenhouse gas management.  More importantly, students going through the proposed 5-year NSERC program will be provided with a multidisciplinary environment for intensive training in discoveries research applicable to their careers (for at least 3 PhD, 6 MSc and 10 BSc).