High resolution gas chromatograph with micro-electron capture/flame ionization detector system (HRGC-uECD/FID)

生态系统受到各种不同的应激源的影响，如有毒污染物、生境改变、入侵物种、气候变暖等，这些都是导致指示物种如运动鱼的生产力和健康下降的原因。正在开发新的工具来补充传统的渔业评估技术，这些技术将能够测量和比较在不同梯度和类型的压力源的环境中鱼类的摄食率和生长转换效率。这两种技术包括：i)使用最先进的有机化学示踪剂的非稳态生物积累模型来跟踪鱼类的摄食率；以及ii)使用脂肪酸信号作为鱼类生长和摄食生态的生物标志物。这项提议要求提供资金，以获得一台带有微型电子捕获检测器和火焰电离检测器的双通道高分辨率气相色谱仪，以支持将验证生态示踪剂等工具在水生生态系统中的使用的校准研究。多氯联苯(PCBS)等有机化学品已分布在全球各地，因此具有潜在的生态示踪剂应用。这些化学品已被证明可跟踪食物网相互作用，并通过测量动物体内浓度随时间的变化，可用于估计动物的喂食率。*所要求的基础设施将提供所需的最灵敏的仪器，以测量从点源移除的系统中的超痕量化学品，如多氯联苯。特定脂肪酸的组织积累，如多不饱和脂肪酸(PUFAs)，已被证明是动物健康和生长的有用指标，最近被用作动物饲养生态的示踪剂。所需的基础设施还将提供高通量仪器，以表征从多个系统收集的鱼类的脂肪酸图谱，满足五大湖环境研究所的三名教员和温莎大学环境工程的一名教员对有机化学和脂肪酸分析的综合需求。

Ecosystems are affected by a wide variety of different stressors such as toxic contaminants, habitat modification, invading species, climate warming ect., that contribute to reductions in the productivity and health of indicators species such as sport fish.  New tools are being developed to supplement traditional fisheries assessment techniques that will enable measurement and comparison of fish feeding rates and growth conversion efficiencies in environments subject to different gradients and types of stressors.  Two such techniques include the i) use of state-of-the-art non-steady state bioaccumulation models of organic chemical tracers to track fish feeding rates and ii) use of fatty acid signatures as biomarkers of fish growth and feeding ecology. This proposal is requesting funding to obtain a duel channel high resolution gas chromatograph with micro electron capture detector and flame ionization detector to support calibration studies that will validate the use of such tools as ecological tracers in aquatic ecosystems. Organic chemicals such as polychlorinated biphenyls (PCBs) have become distributed throughout the globe and thus have potential application as ecological tracers.  These chemicals have been shown to track food web interactions, and by measuring changes in concentration in animals through time, can be used to estimate animal feeding rates.  The requested infrastructure will provide the most sensitive instrumentation available needed to measure ultra-trace levels of chemicals such as PCBs in systems removed from point sources. Tissue accumulation of specific fatty acids, such as polyunsaturated fatty acids (PUFAs), have been shown to be useful indicators of animal health and growth and more recently have been used as tracers of animal feeding ecology. The requested infrastructure will also provide a high throughput instrument to characterize fatty acid profiles in fish collected from multiple systems, meeting the combined needs of organic chemical and fatty acid analyses of three faculty members at the Great Lakes Institute for Environmental Research and one faculty from Environmental Engineering, University of Windsor.