Equipment for behavioural phenotyping of mice

为了进行小鼠行为表型的实验，我们需要三套设备。Tonolab测量大鼠和小鼠的视内压。它被用来检测青光眼，导致视力缺陷。Aimee Wong的博士论文需要这种设备。我们总是对我们的行为研究进行录像，这样第二个观察者就可以重新评分，以确保可靠性，这样我们就可以识别任何实验者的错误。我们使用录像带，但由于数字革命，购买空白录像带变得越来越困难。它也需要很大的空间来存储它们。因此，我们需要使用数码摄像机和计算机与大量的存储和DVD刻录机。我们现有的录像系统和电脑已经使用了10年以上，因此必须更换。这个设备是Timothy O'Leary博士研究所必需的。我们已经开发了一套测试老鼠焦虑、学习和记忆的测试系统，但没有回避条件反射的测试系统。由于许多转基因小鼠具有社会和情感障碍，我们还需要涉及社会行为的学习任务，例如食物偏好的社会传递任务。为了从老鼠身上取出大脑并进行灌注，我们需要新的手术设备。我们现在使用的是中岛信州在1970年代购买的设备，其中大部分需要更换。我们还完成了转基因小鼠大脑的免疫组织化学，这需要一个振动平台来制备抗体。该设备是必要的Leanne Fraser的理学硕士研究。

In order to conduct our experiments on behavioral phenotyping of mice we require three sets of equipment.The Tonolab measures the intra-optic pressure of rats and mice. It is used to detect glaucoma, which results in visual deficits. We require this equipment for the PhD thesis of Aimee Wong.We always videotape our behavioural studies so that the behaviour can be rescored by a second observer to ensure reliability and so that we can identify any experimenter errors. We have used videotapes but because of the digital revolution, it is becoming difficult to purchase blank videotapes. It also takes a lot of room to store them. Thus we are required to use digital video cameras and computers with larger amounts of storage and DVD burners. Our present video system and computers are over 10 years old and therefore must be replaced. This equipment is necessary for Timothy O'Leary's PhD research.We have developed a battery of tests for anxiety, learning and memory in mice, but have no test system for avoidance conditioning. Because many transgenic mice have social and affective disorders, we also need learning tasks that involve social behavior, such as the social transmission of food preference task. In order to remove brains from mice and perform perfusions, we require new surgery equipment. We now use equipment purchased by Shinshu Nakajima in the 1970's, and much of it needs to be replaced. We are also completing immuno-histochemistry on the brains of transgenic mice, which requires a shaker platform to prepare the antibodies. This equipment is necessary for Leanne Fraser's MSc research.