Viral IRES-mediated translation under stress conditions

应激被定义为任何不利的刺激，无论是身体上的、精神上的还是情感上的、内部的或外部的，往往会扰乱生物体的动态平衡，并引发补偿性的生物反应。在我们的现代社会，我们生活在各种压力下，但我们以某种方式适应并继续我们的生活。同样，我们体内的细胞可以改变基本功能的模式，以应对一定的压力。蛋白质合成对于细胞结构和功能的完整性是不可或缺的。有一种独特的蛋白质合成方式，在营养饥饿、细胞损伤和细胞死亡时变得活跃。我们认为，该翻译系统在维持应激状态下的细胞内环境平衡方面起着重要作用。我们在这个方案中的目标是回答细胞如何在应激条件下调节独特的蛋白质合成。建议的研究可以很容易地为一到两个研究生提供论文项目。学员将接触到广泛的研究经验，如分子生物学、细胞生物学和蛋白质生物化学。在拟议的研究计划中培训的高素质人员对加拿大科学界未来将具有重要意义，如学者和卫生研究机构、生物技术公司和政府机构。

Stress is defined as any adverse stimulus, physical, mental or emotional, internal or external, that tends to disturb the organisms homeostasis and elicit compensating biological reactions. In our modern society, we live under various stress but we somehow adapt and go on with our lives. Likewise, cells within our bodies can change the modes of essential function in reaction to certain stress. Protein synthesis is indispensable for the integrity of cellular structure and function. There is a unique way of protein synthesis which becomes active under nutrient starvation, cell injury and cell death. We believe that this translation system plays important roles to maintain cellular homeostasis under stress. Our goal in this proposal is to answer how cells regulate the unique protein synthesis under stress conditions. The proposed study can readily provide thesis projects for one or two graduate students. The trainees will have exposure to a broad range of research experiences, such as molecular biology cellular biology and protein biochemistry. The highly Qualified Personnel trained in the proposed research program will be of great significance to the Canadian scientific community in the future, such as academics and health research institutions, biotechnology companies and government agencies.