Plate reader - Bamfield marine sciences centre

班菲尔德海洋科学中心（BMSC）支持来自40多个加拿大，美国和国际机构的访问科学家的研究，他们研究广泛的学科，包括比较生理学，分子和细胞生物学，生态学和进化生物学，神经科学，入侵物种生物学，发育和生殖生物学以及保护生物学的海洋方面。 利用BMSC的实验室生物学家面临一个严重的问题：将专业设备从他们的家庭实验室运送到BMSC是不切实际的或昂贵的。 此外，除了剥夺研究人员在其本国实验室的使用权外，过去将设备运往BMSC的一些尝试还造成了无法弥补的损坏或昂贵的维修费用。 BMSC越来越多地成为加拿大比较生理学家的基地，因为它拥有强大的研究设施，高质量的流动海水，以及容易获得各种各样的研究动物。 这个不断增长和高度合作的研究人员群体的研究活动越来越受到缺乏自动化设备的限制，大多数人在他们的家庭实验室。 因此，对自动化微板读取器/洗涤器/荧光系统存在以下要求：1）支持多种代谢物浓度和酶活性测定，2）通过自动化板洗涤系统促进更快速和有效的ELISA测定，以及3）使用多种荧光团测量激发/发射波长。 进行此类测定的能力将极大地帮助研究广泛主题的研究人员，包括：摄食、运动和应激对鱼类代谢和呼吸生理的影响，潮间带鱼类对氨和空气暴露的耐受性，原始脊椎动物和广盐鱼类血室酸碱调节和离子调节的进化，社会和生殖生理，硬骨鱼，板鳃类，以及各种无脊椎动物

The Bamfield Marine Sciences Centre (BMSC) supports the research of visiting scientists from over 40 Canadian, U.S. and international institutions who study a wide range of subjects, including marine aspects of comparative physiology, molecular and cell biology, ecology and evolutionary biology, neuroscience, invasive species biology, developmental and reproductive biology, and conservation biology.  Laboratory biologists who utilize BMSC face an acute problem:  it is impractical or expensive to ship specialized equipment from their home laboratories to BMSC.  Furthermore, in addition to depriving research personnel in their home laboratories of its use, some past attempts to ship equipment to BMSC have resulted in irreparable damage or expensive repair bills.  Increasingly, BMSC is becoming a base to a broad range of Canadian comparative physiologists because of the potent mixture of research facilities, high-quality running seawater, and easy access to a great variety of research animals.  The research activities of this growing and highly collaborative group of researchers are increasingly constrained by lack of access to automated equipment that most have in their home laboratories.  A requirement therefore exists for an automated microplate reader/washer/fluorescence system:  1) to support a wide variety of metabolite concentration and enzyme activity assays, 2) to facilitate much more rapid and efficient ELISA assays via an automated plate washing system, and 3) to measure excitation/emission wavelengths using several varieties of fluorophores.  The ability to do such assays will greatly aid researchers who study a wide range of subjects including:  effects of feeding, exercise and stress on fish metabolic and respiratory physiology, tolerance of ammonia and air-exposure in intertidal fish, evolution of blood compartment acid-base regulation and ionic regulation in primitive vertebrates and in euryhaline fish, social and reproductive physiology, and metal toxicity in teleosts, elasmobranchs, and assorted invertebrates.