Spectrophotometric/fluorometric plate reader for metabolic assays

该应用程序是用于肌肉和血液代谢物测定的分光光度/荧光酶标仪。这项RTI补助金的申请人是大卫J.戴克博士（主要申请人）和劳伦斯L。Spriet（共同申请人）。Dyck和Spriet博士都研究哺乳动物骨骼肌中碳水化合物和脂质代谢的调节，目前都持有NSERC发现赠款。 此外，Dyck博士持有CIHR赠款。Dyck博士的研究项目的主要目标是了解骨骼肌中脂肪酸（FA）代谢的基本调节。具体地，这些目标包括i）Ca 2+、能量电荷和AMP活化的蛋白激酶; ii）激素，包括瘦素、脂联素、β-内酰胺酶、IL-6、TNF α;和iii）肥胖症/糖尿病中改变的FA代谢的作用。Spriet博士研究了人体骨骼肌中脂肪和碳水化合物代谢的调节，重点是运动和疾病（肥胖）情况。总的来说，这些研究人员的项目需要对与碳水化合物和脂质代谢相关的肌肉和血液代谢物进行广泛的分析-这些是我们所有研究的核心测量。

This application is for a spectrophotometric/fluorometric plate reader to be used for muscle and blood metabolite assays. The applicants for this RTI grant are Drs. David J. Dyck (principal applicant) and Lawrence L. Spriet (co-applicant). Both Drs. Dyck and Spriet examine the regulation of carbohydrate and lipid metabolism in mammalian skeletal muscle, and each currently hold NSERC Discovery grants.  In addition, Dr. Dyck holds a CIHR grant. The main objective of Dr. Dyck's research program is to understand the basic regulation of fatty acid (FA) metabolism in skeletal muscle. Specifically, these objectives include the role of i) Ca2+, energy charge and AMP-activated protein kinase; ii) hormones including leptin, adiponectin, resistin, IL-6, TNFa; and iii) altered FA metabolism in obesity/diabetes. Dr. Spriet examines the regulaton of fat and carbohydrate metabolism in human skeletal muscle, with emphasis on both exercise and disease (obesity) situations. Collectively, projects from these investigators require the extensive analyses of muscle and blood metabolites related to carbohydrate and lipid metabolism - these are core measurements in all of our studies.