Production of recombinant proteins in seed coats of oil seed crops

加拿大在两种主要油料作物油菜籽和亚麻籽亚麻的生产方面处于世界领先地位。尽管这两种作物对加拿大经济做出了重大贡献，但它们的加工过程表明，生物材料的利用并不理想：油是通过压碎种子生产的，剩余的种子壳大部分作为废弃副产品处理掉。我们建议通过在种皮表皮细胞中生产新的生物制品，将这些废弃的壳转化为有价值的副产品，从而提高这些主要油籽作物的经济价值。这些粘液分泌细胞（MSCs）产生大量的胶质粘液，这些粘液在水合作用后释放出来，使这些细胞成为很好的生物工厂，从中可以很容易地分离出新的产品。为了利用这些生物工厂，我们将首先在拟南芥中测试并优化靶向MSCs的染色质蛋白，然后分别与Performance Plants和Agriculture and agricultural - foods Canada合作，在油菜籽和亚麻籽亚麻中表达蛋白质。这些染色质蛋白构建物将作为一种容易看到的标记物直接应用于检测非转基因批次中转基因种子的不确定存在，这是一个重要的生物安全问题。我们的实验将包括种皮特异性启动子的鉴定和构建，这将为研究界提供组织特异性表达和种皮内产品的遏制资源。我们的工作将支持生物经济和加拿大目前的研究重点，通过为关键生物炼制作物的新型副产品生产提供平台。最后，这种学术、工业和政府研究实验室之间的合作将导致人才的培养，这些人才将通过在研究、工业或公共政策方面的工作，为生物经济做出重大贡献。

Canada is a world leader in the production of the two key oilseed crops Canola and linseed flax. Despite their significant contributions to the Canadian economy, the processing of both crops demonstrates sub-optimal use of the biological material: oil is produced through the crushing of seeds with the remaining seed hulls being largely disposed of as waste by-products. We propose to enhance the economic value of these key oilseed crops by converting these waste hulls to valuable co-products through the production of novel bioproducts in the seed coat epidermal ceils. These mucilage secretory cells (MSCs) produce massive quantities of pectinaceous mucilage that is released following hydration, making these cells excellent biofactories from which novel products can be easily isolated. In order to harness these biofactories, we will first test and optimize chromoprotein targeting to the MSCs in Arabidopsis, then express the proteins in Canola and linseed flax in collaboration with Performance Plants and Agriculture and Agri-Foods Canada, respectively. These chromoprotein constructs will have direct application as an easily visible marker for detecing adventitious presence of transgenic seeds in non-transgenic lots, an important biosafety concern. Our experiments will include the identification and construction of seed coat-specific promoters that will serve as a resource for the research community for tissue-specific expression and containment of products within the seed coat. Our work will support the bioeconomy and Canada's current research priorities through providing a platform for the production of novel co-products in key biorefinery crops. Finally, this collaboration between academic, industrial and government research laboratories will lead to the training of personnel who will be well-poised to make significant contributions to the bioeconomy through the pursuit of jobs in research, industry or public policy.