Ultra-centrifuge - Steacie Supplement

目前心理学系还没有超离心机。离我们最近的是生物系（楼下4层，在大楼的隔壁）。他们非常慷慨地让我们使用它，然而，我们发现，随着我们开始研究有助于再巩固的突触蛋白，我们使用超离心机的频率越来越高。我们认为这一研究领域是我们未来5-10年的重点。鉴于世界上很少有实验室关注杏仁核这个问题，我们正处于一个非常合适的时机，可以发表一些研究，将杏仁核中的受体贩运与行为变化联系起来。这将使我们在这部分战场上处于领先地位。很少有小组能够将行为与突触蛋白联系起来，我们有PKMzeta的数据表明这个项目是可行的。超离心机是实现这一目标的关键。这是唯一的方法，允许定量评估蛋白质在突触后密度，与其他细胞区室污染相对较少。

Currently the Dept of Psychology does not have an ultra-centrifuge. The closest one to us is in the Biology Department (4 floors down and the adjacent wing of the building). They have been very generous in letting us use it, however, we are finding that our use of the ultra-centrifuge is becoming more frequent as we begin to examine synaptic proteins that contribute to reconsolidation. We feel this area of research is the next 5-10 yrs of focus for us. Given that few labs in the world are focused on this issue amygdala, we are in a very opportune moment to publish some studies linking receptor trafficking in the amygdala with changes in behavior. This would place us at the lead of this portion of the field. Few groups have been able to make the link from behavior to synaptic proteins and we have data with PKMzeta indicating that the project is feasible. The ultra-centrifuge is central to make that happen. It is the only method to allow a quantitative evaluation of proteins in the post-synaptic density, with relatively little contamination from other cellular compartments.