Multiwell gamma counter for research in fish endocrinolgy and physiology

本应用程序是用于J. Chang G. Goss和J. Stafford实验室鱼类生理学研究的多探测器伽马计数器。张博士研究金鱼生长激素和促性腺激素释放和合成的控制，重点研究细胞内信号通路如何整合和介导多种神经内分泌调节因子对金鱼促性腺激素和促性腺激素合成和分泌的差异影响。通过基于125i的放射免疫分析法准确定量激素分泌和细胞激素含量的能力对于本研究项目至关重要。戈斯博士研究了淡水和海洋鱼类的离子、流体和酸碱平衡是如何被调节的。他的研究重点包括阐明所涉及的转运蛋白的身份和功能调节，以及这些转运蛋白在不同鳃细胞群上的差异定位如何在正常环境挑战和暴露于水性药物和纳米颗粒时维持体内平衡。其中一个关键问题是Na+通量和Na+转运体系统的调控。监测22Na通量的能力对此类研究至关重要。斯塔福德博士研究调节鱼类先天免疫反应的受体配体系统，以及这些成分如何调节细胞毒性淋巴细胞消除感染细胞的能力。51Cr释放试验是监测他的研究所需的细胞毒性淋巴细胞反应的标准技术。我们用了18年的伽马计数器已经不能正常工作了。它们也不能很好地监测Na和Cr的同位素。申请的Perkin Elmer Wizard 2470具有处理Chang实验室所需的大量激素分析的能力，并且还设计用于监测22Na和51Cr。用申请的计数器替换当前的计数器对于三个高效研究项目的运作和持续成功是绝对必要的，并且是迫切需要的。

This application is for a multi-detector gamma counter for use in fish physiology research in the laboratories of J. Chang G. Goss and J. Stafford. Dr. Chang studies the control of growth hormone and gonadotropin release and synthesis in goldfish with emphasis on how intracellular signalling pathways integrate and mediate the differential effects of multiple neuroendocrine regulators on hormone synthesis and secretion in goldfish gonadotropes and somatotropes. The ability to accurately quantify hormone secretion and cellular hormone content by 125I-based radioimmunoassay is essential for this research program. Dr. Goss investigates how ionic, fluid and acid/base balance in freshwater and marine fish species are regulated. His research focuses include elucidating the identity and functional regulation of transporters involved, as well as how the differential localization of these transporters on different gill cell populations contribute to the maintenance of homeostasis under normal environmental challenges and when exposed to water-borne pharmaceuticals and nanoparticles. One key question is the regulation of Na+ flux and Na+ transporter systems. The ability to monitor 22Na flux is critical for such studies. Dr. Stafford studies the receptor-ligand systems regulating the innate immune response in fish and how these components regulate the ability of cytotoxic lymphocytes to eliminate infected cells. A 51Cr release assay is a standard technique to monitor cytotoxic lymphocyte responses required for his research. Our 18-yr old gamma counters are no longer functioning reliably. They also do not properly monitor Na and Cr isotopes. The Perkin Elmer Wizard 2470 applied for has the capacity to process the large number of hormone assays required for the Chang lab and is also designed to monitor 22Na and 51Cr. The replacement of the current counters with the one applied for is absolutely essential for the functioning and continual success of three highly productive research programs and is urgently needed.