A UHPLC system for complex extracts in agricultural, food and nutritional research

我们的食品、营养和农业研究项目所获得的天然提取物中高度复杂的化合物混合物的分析需要一种能够实现最高可用分离能力的超高压液相色谱(UHPLC)系统来辅助分析。该装置配备了二极管阵列(DAD)和蒸发光散射(ELSD)检测器，用于识别和定量各种分析物。ELSD针对低温操作进行了优化，这将特别有利于涉及血脂(Curtis、Gänzle和field)的研究。一个完整的组分收集系统将允许在适合通过质谱分析或生物活性测试进一步分析的范围内自动收集峰。便携式配置既允许独立操作，也允许与相邻的质谱仪耦合，从而最大限度地发挥省级和联邦政府对这一昂贵基础设施的投资的好处。UHPLC系统将立即被从事当前项目的学生和同事使用。其中许多项目旨在开发有利于加拿大农业和食品加工商的附加值产品。这些例子包括：表征可控制食品真菌腐败的羟基化脂肪酸(Gänzle，Curtis)、分析天然保健品中的酚类化合物及其与食品蛋白质的反应(Schieber)、分析油菜籽精炼废物除臭馏出物中有价值的脂肪残留(Temelli，Curtis)、三酰甘油酯的分析和植物油转化的定量(Curtis)、分析肿瘤膜和脂筏中的神经酰胺(field，Curtis)、分析从作物生物质中提取的生物活性化合物(Aranda Saldana)。总体而言，UHPLC系统将是一个主要的工具，将促进强有力的合作研究。

An ultra-high pressure liquid chromatography (UHPLC) system capable of achieving the highest available separation power is requested to aid in the analysis of highly complex mixtures of compounds in natural extracts obtained by our research programs in food, nutrition and agriculture. The unit is equipped with diode-array (DAD) and evaporative light scattering (ELSD) detectors for identification and quantification of a wide range of analytes. The ELSD is optimized for low temperature operation which will especially benefit studies involving lipids (Curtis, Gänzle and Field). An integral fraction collection system will allow automated collection of peaks on a scale suitable for further analysis by mass spectrometry or testing for bioactivity. A portable configuration permits both stand-alone operation and coupling to adjacent mass spectrometers, maximizing the benefit of provincial and federal investments in this expensive infrastructure. The UHPLC system will find immediate use by students and associates working on current projects. Many of these projects are aimed a developing value-added products which are beneficial to Canadian agriculture and food processors. Examples include the characterization of hydroxylated fatty acids which may control fungal spoilage of foods (Gänzle, Curtis), analysis of phenolic compounds in natural health products and their reactions with food proteins (Schieber), analysis of valuable lipid residues in waste deodorizer distillate from canola refining (Temelli, Curtis), analytical separations of triacylglycerides and quantification of vegetable oil transformations (Curtis); analysis of ceramides in tumour membrane and lipid rafts (Field, Curtis); analysis of bioactive compounds extracted from crop biomass (Aranda Saldana). Overall the UHPLC system will be a workhorse instrument which will facilitate strong collaborative research.