Shared instruments (luminometer and real-time thermocycler) for gene expression analysis

基因表达分析在细胞和生物化学研究中起着至关重要的作用。虽然经典的检测方法仍然被接受，但研究人员和科学期刊已经广泛和压倒性地要求现代和高灵敏度的技术。为了保持我们在研究和培训方面的竞争力，我们正在寻求财政支持，以购买高灵敏度的发光计（Turner Biosystems公司的20/20n管发光计）和实时热循环仪（Applied Biosystems公司的StepOnePlusTM）。这些最先进的仪器是必不可少的，将被化学和生物化学系（温莎大学）的六个研究小组大量使用。所要求的光度计将广泛用于双荧光素酶报告分析，以确定启动子和调控元件的功能；而实时热循环仪将广泛用于定量分析，包括基因分型和基因表达谱。目前该部没有类似的制度。这些仪器的收购将对正在进行和新开发的研究项目的质量和生产力以及高素质人才的培训产生巨大而积极的影响。

Gene expression analysis plays a pivotal role in cell and biochemical researches. While classical detection methods are still accepted, modern and highly sensitive techniques have been widely and overwhelmingly demanded by researchers and scientific journals. To maintain our competitiveness in research and training, we are seeking a financial support for the acquisition of a highly sensitive luminometer (20/20n Tube Luminometer by Turner Biosystems) and a real time thermocycler (StepOnePlusTM by Applied Biosystems). These state-of-the-art instruments are essential and will be heavily used by six research groups in the Department of Chemistry and Biochemistry (University of Windsor). The requested luminometer will be extensively used for dual luciferase reporter assays to determine the functions of promoters and regulatory elements; while the real time thermocycler will be extensively used for quantitative analyses, including genotyping and gene expression profiling. Currently there is no equivalent system available in the Department. The acquisition of these instruments will have tremendous and positive impact on the quality and productivity of the on-going and newly developed research programs, as well as on the training of highly qualified personnel.