Role of phosphatases in neural stem cell differentiation

蛋白酪氨酸磷酸酶(PTPs)在整个动物界的细胞中都有表达。PTPs负责从蛋白质中的氨基酸酪氨酸中去除磷酸基团。它们对于逆转蛋白质中磷酸基团的添加作为细胞中的一种调节机制是重要的。PTPs对哺乳动物神经系统的发育和功能的贡献几乎是未知的。这项研究拨款的目的是研究PTPs在神经系统早期发育中的作用。我们将研究从小鼠大脑中的干细胞形成新细胞的方面，这些干细胞已经被基因工程改造为产生不活跃的PTP。实验将完成，以确定PTP如何影响大脑发育过程中细胞的分裂和细胞死亡。这些实验很重要，因为这些信息将告诉我们控制大脑中新细胞产生的生物途径。这项拨款的结果对刺激受疾病或损伤影响的大脑新生长的疗法的进展具有更广泛的影响。

Protein tyrosine phosphatases (PTPs) are expressed in cells throughout the animal kingdom. PTPs are responsible for removing phosphate groups from the amino acid tyrosine in proteins. They are important to reverse the addition of phosphate groups to proteins as a regulatory mechanism in the cell. The contribution of PTPs to the development and function of the mammalian nervous system are virtually unknown. The purpose of this research grant is to study the role of PTPs during the early development of the nervous system. We will study aspects of new cell formation from stem cells in the brains of mice that have been genetically engineered to produce inactive PTP. Experiments will be completed to determine how PTP affects the division of cells and the death of cells during brain development. These experiments are important because the information will inform us of the biological pathways that control the production of new cells in the brain. The results of this grant have broader implications for progress toward therapies that stimulate new growth in the brain affected by disease or injury.