Elucidation of endopolygalacturonase structural determinants
Elucidation of endopolygalacturonase structural determinants
批准号:
138654-2008
负责人:
Beauregard, Marc
金额:
$1.75万
依托单位国家:
加拿大
项目类别:
Discovery Grants Program - Individual
财政年份:
2009
资助国家:
加拿大
项目状态:
已结题
起止时间:
2009-01-01 至 2010-12-31
中文摘要
对于几个行业来说,酶正成为越来越重要的产品。它们对环境是安全的,比以往任何时候都便宜,在最佳条件下使用时,比化学催化剂效率高得多。随着蛋白质工程和定向进化的最新进展,定制的酶将可用于天然酶不适合的工业过程。作为工业生物催化剂,酶功效的核心是稳定性问题。事实上,酶必须经受相当恶劣的条件,才能提供污染化学品的经济替代品;因此,为工业应用定制酶将需要深入了解蛋白质中的稳定性决定因素。
英文摘要
Enzymes are becoming increasingly important products for several industries. They are safe for the environment, cheaper than ever, and, when used under optimal conditions, much more efficient than chemical catalysts. With recent advances in protein engineering and directed evolution, customized enzymes will become available for industrial processes for which the native enzymes were unsuitable. At the heart of enzyme efficacy as industrial biocatalysts are stability issues. Indeed, enzymes have to stand rather harsh conditions in order to provide economical alternatives to polluting chemicals; customizing enzymes for industrial application will therefore require an in-depth understanding of stability determinants in proteins.
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