Diluters or extenders for mammalian sperm processing and preservation

公牛精液冷冻保存是继人工授精技术问世后，奶牛养殖业取得的最重要成果之一。这两种方法使所需基因系能够以合理的成本在全球范围内分布和使用。加拿大占全球经济总量的3%，但却提供了全球牛精液出口的20%(约7000万美元/年)。卵黄和牛奶是保存精子最常用的保护剂。安永和牛奶是动物来源的产品，存在着精液污染的潜在风险。因此，迫切需要开发不含动物来源产品的新介质来保存精液。然而，很难找到安永或牛奶的替代成分，因为安永和牛奶保护精子的机制尚不清楚。我们的工作为EY保护精子的机制提供了新的见解。我们发现，所有有蹄类动物的精囊都被研究成

The cryopreservation of bull semen represents one of the most important achievements in dairy farming after the introduction of artificial insemination. These two approaches have enabled the worldwide distribution and use of desired genetic lines at a reasonable cost. Canada represents <3% of the global economy but provides >20% of world bovine semen exports (~70 million dollars/annum). Egg yolk (EY) and milk are the most used protective agents to conserve sperm. EY and milk, being products of animal origin, represent a potential risk of contamination of semen. Thus, there is an urgent need of developing novel media free of products from animal origin to conserve semen. However, it is difficult to find replacement component for EY or milk because the mechanisms involved in sperm protection by EY and milk remain unclear. Our work has provided new insight into the mechanism of sperm protection by EY. We showed that seminal vesicles of all ungulates studied to