Automated instrument for timecourse metabolic studies of bacteria. Systeme automatise pour l'etude dynamique du metabolisme bacterien.

研究细菌对环境变化的适应性需要严格的培养条件。在我们的实验室中，我们使用生物反应器（发酵罐）来实现这一目标，它具有允许控制和监测许多物理化学参数的额外优势，这使我们能够产生否则无法达到的培养条件。我们研究的细菌在健康（霍乱弧菌，金黄色葡萄球菌），生物技术（链霉菌属）和环境（固氮的法兰克菌属）领域具有极大的兴趣。其中一些在后期培养中也具有突然休眠的特征，值得研究它们适应环境压力的机制。要求的自动采样和分析仪器将使我们的研究扩展到新的领域，利用蛋白质组学和转录组学策略来更好地了解水平基因转移如何转化为霍乱弧菌的生态适应性，以及环境条件如何影响链霉菌，金黄色葡萄球菌，所要求的仪器将允许连续取样和监测连续生物反应器培养中的营养物和代谢物，否则这是不可行的。它将是培养微生物学和生物技术方面的高素质人员的中心。

The study of bacterial adaptation to environmental change requires rigorous culture conditions. In our laboratories, we achieve this using bioreactors (fermentors) which have the added advantage of allowing the control and monitoring of many physico-chemical parameters, which enable us to produce culture conditions unattainable otherwise. The bacteria we study are of utmost interest in the fields of health (Vibrio cholerae, Staphylococcus aureus), biotechnology (Streptomyces spp.), and environment (the nitrogen-fixing Frankia sp.). Some of these share also the characteristics of sudden dormancy in late-phase cultures and warrant research in to their mechanisms of adaptation to environmental stress. The automated sampling and analysis instrument requested will allow us to expand our research into new horizons, exploiting proteomic and transcriptomic strategies to better understand how horizontal gene transfer translates into ecological fitness in Vibrio cholerae, and how environmental conditions affect the metabolism in Streptomyces spp., S. aureus, and Frankia sp. The instrument requested will allow the continuous sampling and monitoring of nutrients and metabolites in continuous bioreactor cultures which would not be feasible otherwise. It will be central to the training of highly qualified personnel in microbiology and biotechnology.