A preparative FPLC for biomolecular research

本提案的目的是获得制备型快速蛋白液相色谱（FPLC）仪器，用于物理和化学部门的生物大分子纯化和表征。FPLC是蛋白质、多糖和其他生物大分子高效、规模化纯化的重要实验技术。生物物理学、生物分析化学、生物材料和化学生物学中越来越多的跨学科研究项目需要这种仪器，这些项目需要一种简便且可扩展的生物分子纯化方法。GE医疗要求的FPLC是一种强大的、通用的系统，可以与各种色谱介质一起使用，以纯化这些项目所需的大量生物分子。除了对本提案中描述的具体研究项目的重要影响外，该FPLC系统的收购将通过为HQP培训提供重要资源，加强理学院、环境基因组学高级研究中心和NSERC CREATE“定量生物医学”和“药物化学和生物制药开发”培训项目的跨学科研究和培训工作。

The aim of this proposal is to acquire preparative fast protein liquid chromatography (FPLC) instrumentation for biological macromolecule purification and characterization in the departments of Physics and Chemistry. FPLC is an essential experimental technique for efficient and scalable purification of proteins, polysaccharides, and other biological macromolecules. This instrumentation is required for a growing number of interdisciplinary research programs in biophysics, bioanalytical chemistry, biomaterials, and chemical biology that require a facile and scalable method of biomolecular purification. The requested FPLC from GE Healthcare is a robust, versatile system that can be used with a wide range of chromatography media to purify biomolecules in the large-scale quantities required for these programs. Beyond its important impact on the specific research programs described in this proposal, acquisition of this FPLC system will enhance interdisciplinary research and training efforts within the Faculty of Science, the Centre for Advanced Research in Environmental Genomics, and the NSERC CREATE training programs in "Quantitative Biomedicine" and "Medicinal Chemistry and Biopharmaceutical Development" by providing an important resource for the training of HQP.