Cellular mechanisms of microbial-epithelial interactions

肠道功能障碍严重影响农业和人口。肠道疾病和腹泻通常是由于暴露于各种微生物病原体，包括原生动物寄生虫贾第虫属引起的疾病通常被称为“海狸热”。充分的证据表明，贾第虫是伴侣动物和幼龄牲畜最常见的寄生虫之一，它有很大的传播给人类的潜力。贾第虫是儿童和旅行者腹泻的常见原因。宿主与微生物之间的相互作用决定了感染的结果，但这一点仍不完全清楚。贾第虫病中导致肠吸收不良和腹泻的上皮异常与在其他肠道疾病中观察到的上皮异常相似，如细菌性肠炎、慢性食物过敏反应、克罗恩病和乳糜泻。因此，更好地了解贾第鞭毛虫病的这些过程可能有助于解开各种肠道疾病的机制。利用互补的体外和体内贾第虫挑战模型系统，该建议建立在有希望的初步研究结果，以确定在肠道微生物-上皮相互作用的基本机制。

Intestinal malfunction severely affects the agricultural industry as well as the human population. Intestinal disease and diarrhea often results from exposure to a variety of microbial pathogens, including the Protozoan parasite Giardia sp. The disease caused by Giardia is commonly referred to as "beaver fever". Ample evidence suggests that Giardia is one of the most common parasite of companion animals and young livestock, and it has great potential for transmission to humans. Giardia is a very common cause of diarrhea in children and in travelers. The host-microbial interactions that govern the outcome of infection remain incompletely understood. The epithelial abnormalities responsible for intestinal malabsorption and diarrhea in giardiasis share similarities with those observed in other enteric disorders, such as bacterial enteritis, chronic food anaphylaxis, Crohn's disease, and celiac disease. Therefore, a better understanding of these processes in giardiasis may help unravel mechanisms implicated in a variety of intestinal diseases. Using complementary in vitro and in vivo Giardia challenge model systems, this proposal builds upon promising preliminary findings to identify basic mechanisms involved during microbial-epithelial interactions in the gut.