Evaluation of inactivation efficacy of viral pathogens using integrated biowaste utilization system-pathogen destruction unit (IBUS-p)

将病毒在培养细胞中的生长与实时聚合酶链式反应(ICC-RTPCR)相结合，建立了一种新的检测方法。这种方法可以检测饮用水和废水样本中的病毒，并确定病毒是否活着和具有传染性。ICC-RTPCR是一种非常灵敏的细胞培养方法，比美国环保局推荐的现行细胞培养方法要好得多。使用这种方法，我们在处理后的废水中发现了与人和动物腹泻有关的活病毒。毫无疑问，该技术在饮用水和环境地表水监测中得到批准，将有助于促进饮用水安全和公众健康。

A novel detection method has been developed by integrating virus growth in cultured cells and real time polymerase chain reaction (ICC-rtPCR). This method could spot the viruses in drinking water and wastewater samples, and identify whether the virus is alive and infectious as well. ICC-rtPCR is very sensitive and much better than current cell culture method recommended by US Environment Protect Agency. Using this method, we have found living viruses associated with human and animal diarrhea in the treated wastewater. Undoubtedly, we can promote drinking water safety and public health when this technology is ratified in monitoring drinking water and environmental surface water.