Ozone free box for microarray based functional genomics studies

高通量功能基因组学和蛋白质组学研究是农业、食品和营养科学系多用户农业基因组学和蛋白质组学单位（AGP单位）研究的主要重点。来自艾伯塔省牲畜基因组学独创性中心、乳制品营养研究小组、猪繁殖发育计划/胚胎基因网络、植物生物技术和植物病理学小组、人类营养计划、免疫学、食品微生物学、功能食品和营养保健品小组的各种研究人员，以及加拿大农业和农业食品部的科学家，目前正在支持100多个用户的研究项目。所有这些团体都在进行创新努力，以提高牲畜的产量和繁殖性能，开发营养品质更好的动物产品，了解宿主-微生物相互作用的分子机制，并创造具有更好抗病性和增值特性的作物。DNA和蛋白质微阵列被AGP单位的大多数研究人员广泛使用。BioTray®的Ozone Free BoxTM是一种强大的解决方案，完全破坏臭氧，以便在微阵列样品制备，杂交，洗涤，干燥和扫描阶段具有无臭氧环境。有了这个无臭氧空间，微阵列将以最小的技术变化产生高度可重复的数据，这对于在不同处理或生理条件下准确量化给定细胞或组织中的转录本尤为重要。这也将显著提高扫描微阵列载玻片的图像质量，增加扫描后的再现性和数据质量。微阵列杂交站的可用性是至关重要的，并且需要在这个大型核心设施内的9个主要用户以及其他次要用户的研究项目的各种应用。这将进一步促进当前基因组学技术的学生、技术人员和博士后的培训。该基础设施支持的研究将对加拿大农业的未来可持续性和盈利能力发挥重要作用。

High throughput functional genomics and proteomics studies are the major focus of the research conducted in a multi-user Agricultural Genomics and Proteomics Unit (AGP Unit) in the Department of Agricultural, Food and Nutritional Science. Various researchers from The Alberta Ingenuity Centre for Livestock Genomics, The Dairy Nutritional Research Group, The Swine Reproduction-Development Program/Embryogene Network, Plant Biotechnology and plant pathology group, Human Nutrition program, Immunology, Food microbiology, Functional Foods and Nutraceuticals group, and scientists at Agriculture and Agri-Food Canada, are currently supporting the research programs of over 100 users. All these groups are applying innovative efforts to improve production and reproductive performance of livestock, developing animal products with improved nutritional qualities, understanding molecular mechanisms of host-microbial interactions and creating crops with improved disease resistance and value added characteristics. DNA and protein microarrays are widely used by most of the researchers at the AGP Unit. BioTray®'s Ozone Free BoxTM is a powerful solution which totally destroys ozone in order to have an ozone free environment during microarray sample preparation, hybridization, washes, drying and scanning stages. With this ozone free space, highly reproducible data from microarray with minimal technical variation will be generated and this is particularly important to accurately quantify the transcripts in a given cell or tissue under different treatments or physiological conditions. This should also significantly improve image quality of the scanned microarray slides with increased reproducibility and data quality after scanning. The availability of the microarray hybridization station is crucial and required for a variety of applications by research projects of 9 major users as well as additional minor users within this large core facility. This will further facilitate the training of students, technicians and postdocs in current genomics technologies. The research supported by this infrastructure will play an important role towards the future sustainability and profitability of Canadian agriculture industries.