Purification system for the isolation of ribonucleoprotein components

为生物学上重要的分子定义新功能的关键一步是能够从细胞中存在的大量其他分子中特异性地分离或分离这些分子。这对研究人员来说是一项极具挑战性的任务，因为在许多情况下，你感兴趣的特定分子可能只存在于非常小的数量中。专门的研究设备，如液相色谱系统，需要执行这些高分辨率的生物分离。这种设备是专门设计用来探测不同大分子之间物理和化学性质的细微差别的。高效液相色谱纯化系统将为我的课课组提供纯化细胞中具有基本功能的蛋白质- rna复合物的能力。它还将为莱斯布里奇大学的其他研究人员提供分离、操作、量化和识别其他类型生物分子的能力。仅在生物学系，该纯化系统也将被用作研究癌症生物学中DNA损伤后果的工具，并研究模式植物物种中调节叶片发育的重要蛋白质。该设备将为学生提供宝贵的培训和专业知识，使用最先进的蛋白质纯化设备，适用于众多科学学科。

A critical step towards defining new functions for biologically important molecules is the ability to specifically isolate or separate these molecules from the large number of other molecules that are present in a cell. This can be an extremely challenging task for researchers because in many cases the specific molecule that you are interested in studying may only be present in very small quantities. Specialized research equipment, such as a liquid chromatography system, is required to perform these high-resolution biological separations. This equipment has been specifically designed to exploit the subtle differences in physical and chemical properties between different macromolecules. A high-performance liquid chromatography purification system will provide my research group with the ability to purify protein-RNA complexes which have essential functions in cells. It will also provide other researchers at the University of Lethbridge with the ability to separate, manipulate, quantify, and identify other types of biological molecules. Within the biology department alone, this purification system will also be used as a tool to investigate the consequences of DNA damage in cancer biology and study the important proteins that regulate leaf development in model plant species. This equipment will provide students with invaluable training and expertise in using state-of-the art protein purification equipment applicable to a multitude of scientific disciplines.