From source to sink - carbon allocation in poplar

像所有的植物一样，树木不断地监测内源和环境线索，并利用这些信息来调整资源分配，以最大限度地获得资源，并最大限度地减少对有害现象的暴露。为了做到这一点，树木/植物拥有整合和解释由内部和环境信号提供的信息的机制。最终，这些现象在基因表达水平上受到控制，从而表现为各种可溶性代谢物的积累，包括碳水化合物。深入了解树木整个生命周期中控制蔗糖生物合成(分解代谢和合成代谢)的复杂生物合成过程，对于了解细胞程序性生长模式、对环境提示的反应(S)、抵御环境胁迫(生物和非生物)的能力以及细胞壁发育的复杂网络和调控框架(形态、超微结构和化学)至关重要。本研究试图阐明蔗糖在树木，特别是杨树中合成和去向(即分配和分配)的控制机制。这一目标将通过对蔗糖代谢的长期、机械性功能基因组学研究来实现，同时结合旨在阐明关键代谢物和调节碳水化合物代谢的基因的平行生化(代谢物图谱)和分子(转录调控)研究。利用一系列功能基因组学技术，结合传统的细胞壁生物化学，研究杨树资源分配的复杂性(积累、降解和组织特异性定位)，包括蔗糖的生物合成和转运、淀粉的生成(包括其动员)和低聚碳水化合物(如棉籽糖、水苏糖)的生物合成。

Like all plants, trees constantly monitor endogenous and environmental cues, and use this information to make adjustments in resource allocation, both to maximize resource acquisition and to minimize exposure to deleterious phenomena. To accomplish this, trees/plants possess mechanisms that integrate and interpret the information provided by internal and environmental signals. Ultimately, these phenomena are controlled at the level of gene expression, which consequently manifests the accumulation of a variety of soluble metabolites, including carbohydrates. A thorough understanding of the intricacies of the biosynthetic processes governing sucrose biosynthesis (catabolism and anabolism) throughout the life cycle of trees is pivotal to understanding programmed cellular growth patterns, response(s) to environmental cues, the ability to withstand environmental stresses (biotic and abiotic), and the complex network and regulatory frameworks fundamental to the development of cell wall (morphology, ultrastructure and chemistry). This research project will attempt to elucidate the control mechanisms that underpin the synthesis and fate (i.e. allocation and partitioning) of sucrose in trees, specifically Populus. This objective will be achieved by employing a long-term, mechanistic functional genomics investigation of sucrose metabolism, combining parallel biochemical (metabolite profiling) and molecular (transcriptional regulation) studies aimed at elucidating the key metabolites and genes modulating carbohydrate metabolism. The complexities (accumulation, degradation, and tissue specific localization) of "resource allocation", including sucrose biosynthesis and translocation, the generation of starch (including its mobilization), and the biosynthesis of oligomeric carbohydrates (i.e. raffinose, stachyose) will be investigated in Populus, employing an array of functional genomics techniques coupled with traditional cell wall biochemistry.