Artificial Sweetener? A Functional Genomics Interrogation of the Impact of Soluble Carbohydrate Metabolism on Poplar Cell Wall Biosynthesis

人造甜味剂？

• 批准号: 238354-2012
• 负责人: Mansfield, Shawn
• 金额: $ 4.08万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=546964
• 关键词: Artificial; Sweetener; Functional; Genomics; Interrogation

Long-lived organisms, such as trees, offer a unique system to investigate how plants allocate carbon, as their perennial nature permits an interrogation of the complex responses to variations in environmental cues while avoiding the dilution of phenotypic responses that arise from genetic segregation common to annual plants. Trees, like all plants, possess an inherent plasticity in their ability to allocate carbon, which is pivitol to the effective modulation of growth and development. Ultimately, resource allocation is controlled by gene expression, which translates into the accumulation of a variety of soluble metabolites, including carbohydrates and phenolics. The resulting chemistry defines the physiology and, importantly, impacts plant form, function and fitness. The long-term objectives of the Mansfield research program, is to understand the complex fundamental processes of carbon allocation in Populus, with a specific emphasis on the intricacies of the biosynthetic processes governing soluble carbohydrate metabolism and how these metabolites contribute to cell wall development. This information is pivotal to understanding programmed cellular growth patterns, and the complex network and regulatory frameworks fundamental to carbon translocation from source tissues to sink tissues, where wood development is a substantial terminal product. The proposed research builds on the previous NSERC Discovery Grants in the Mansfield lab as a foundation to test hypotheses related to the breadth of molecular interactions that shape plant carbon allocation in Populus. Specifically, the proposed research program comprises a body of experiments aimed to address the following objectives: 1) elucidate the nature of the translocatable photoassimilate in Populus; 2) track the fate of carbon supplying the developing xylem of Populus, and 3) dissect the interplay between sucrose synthesis, fructose recycling, and carbohydrate signaling in Populus sink tissue. A greater understanding of the molecular controls that underpin resource allocation in trees could also facilitate strategies aimed at selecting, breeding, and/or engineering trees that maximize resource allocation and strategically direct carbon to vegetative biomass.

长寿的生物体，如树木，提供了一个独特的系统来研究植物如何分配碳，因为它们的多年生性质允许询问对环境线索变化的复杂反应，同时避免一年生植物常见的遗传分离引起的表型反应的稀释。与所有植物一样，树木在分配碳的能力方面具有固有的可塑性，这对于有效调节生长和发育至关重要。最终，资源分配由基因表达控制，基因表达转化为各种可溶性代谢物的积累，包括碳水化合物和酚类物质。由此产生的化学反应定义了生理学，更重要的是，影响植物的形态、功能和适应性。曼斯菲尔德研究计划的长期目标是了解杨树中碳分配的复杂基本过程，特别强调控制可溶性碳水化合物代谢的生物合成过程的复杂性以及这些代谢物如何促进细胞壁发育。这些信息对于理解程序化细胞生长模式以及碳从源组织转移到库组织至关重要的复杂网络和监管框架至关重要，其中木材开发是重要的终端产品。拟议的研究建立在曼斯菲尔德实验室之前的 NSERC 发现资助的基础上，作为测试与影响杨树植物碳分配的分子相互作用广度相关的假设的基础。具体来说，拟议的研究计划包括一系列旨在实现以下目标的实验：1）阐明杨树中可易位光同化物的性质； 2) 追踪为杨树发育中的木质部提供碳的命运，3) 剖析杨树库组织中蔗糖合​​成、果糖回收和碳水化合物信号传导之间的相互作用。 更好地了解支持树木资源分配的分子控制也可以促进旨在选择、育种和/或改造树木的策略，从而最大限度地提高资源分配并战略性地将碳引导至植物生物量。