High Resolution Imaging Suite for Analysis of Development

绘制发育过程和基因表达图的能力需要子宫内以及器官和细胞培养中的高分辨率显微镜。成像的最新进展还有一个优势，那就是除了静态成像外，还能够提供功能数据。这项建议的目的是建立一个显微镜套件，可以用来通过几种不同的方式绘制发育过程和基因表达图。拟议中的设备将为我们提供一种能力，以确定基因随着时间的推移在青蛙和小鼠模型中的表达。通过对整个胚胎进行分析，以及评估单个细胞的表达，宏观荧光显微镜将能够分析转基因动物的荧光基因表达。高端单色数码相机结合高功率物镜和多个荧光滤光片，能够同时评估多个荧光团的亚细胞定位。最后，我们需要一个用于麻醉小鼠的异氟烷室，从而允许通过高分辨率超声波对活体动物的组织发育进行功能分析。通过将这一基础设施与当前的显微镜核心相结合，我们将能够以一种我们以前无法做到的方式来检查发育过程。

The ability to map developmental processes and gene expression requires high resolution microscopy both in utero, as well as in organ and cell cultures. Recent advances in imaging also have an advantage in being able to provide functional data in addition to static imaging. The purpose of this proposal is to establish a microscopy suite that can be used to map developmental processes and gene expression through several different modalities. The proposed equipment will provide us with an ability to identify the expression of genes over time in developing frog and mouse models. A macrofluorescent microscope will enable analysis of fluorescent gene expression in transgenic animals by allowing whole embryo analysis, as well as the ability to assess individual cell expression. A high end monochrome digital camera combined with a high power objective and multiple fluorochrome filters provides the ability to assess subcellular localization of multiple fluorophores at the same time. Finally, we require an isofluorane chamber for anesthetizing mice, thereby allowing for functional analysis of tissue development in live animals by high resolution ultrasound. By combining this infrastructure with the current microscopy core, we will be able to examine developmental processes in a fashion that we have previously be unable to do.