Designer Peptides and Proteins: Characterization by High Pressure Liquid Chromatography

化学生物学的关键是材料的纯度，无论是用于设计新型多肽/蛋白质修饰的水凝胶、药物胶体还是金属螯合聚合物。高压液相色谱仪使生物分子得到提纯，聚合反应动力学得以定量。在Molly Shoichet、Mitchell Winnik和Brian Shoichet的实验室正在进行的高级研究中，高效液相色谱是成功的关键。在联合实验室中，有70名高素质的人员将直接受益于高效液相色谱，另外还有100多名研究人员在我们的合作者实验室中。重要的是，拟议的高效液相色谱法已成为现代实验室的支柱，这反映在来自三个不同学院的主要研究人员提出的多学科研究：应用科学与工程(MS Shoichet)、艺术与科学(MA Winnik)和药学(BK Shoichet)。由于校园内其他高效液相色谱仪器的使用有限，而且实验室在去年夏天旧的高效液相色谱仪失效时几乎处于停顿状态，很明显，高效液相色谱法对研究速度有重大影响。从高效液相色谱获得的信息将使我们能够推动我们的知识型经济，特别是在免疫分析、细胞输送和药物配方领域。这一点从我们与加拿大和世界各地的行业的多个接触点和合作中可见一斑。MA Winnik的金属螯合聚合物已经与一家总部位于安大略省的公司进行了商业化演示；CCRM与一家大型制药公司合作，有权授权MS Shoichet的一项发明，并有兴趣使用这种高效液相，从而进一步加强我们与加拿大行业的联系。

Key to chemical biology is purity of materials, whether for the design of new peptide/protein-modified hydrogels, drug colloids, or metal-chelating polymers. The high pressure liquid chromatograph (HPLC) allows both biomolecules to be purified and polymerization reaction kinetics to be quantified. The HPLC is critical to success in the advanced research being pursued by the laboratories of Molly Shoichet, Mitchell Winnik and Brian Shoichet. In the combined laboratories, there are 70 highly qualified personnel who will benefit directly from the HPLC, plus another 100+ researchers in our collaborators' laboratories. Importantly, the proposed HPLC has become a mainstay of the modern laboratory as reflected in the multi-disciplinary research proposed by principal investigators from three distinct faculties: Applied Science & Engineering (MS Shoichet), Arts & Science (MA Winnik) and Pharmaceutical Sciences (BK Shoichet). With limited access to other HPLC instruments on campus and the near standstill to which the laboratory was taken when an old HPLC was non-functional this past summer, it is clear that the HPLC has a significant impact on the pace of research. The information obtained from the HPLC will allow us to advance our knowledge-based economy, particularly in the fields of immunoassays, cell delivery and drug formulations. This is clear from our multiple points of contact and collaboration with industry in Canada and worldwide. Commercialization of MA Winnik's metal-chelating polymers has been demonstrated with an Ontario-based company; CCRM, in collaboration with a large pharmaceutical company, has an option to license one of MS Shoichet's inventions and is interested in having access to this HPLC, thereby further strengthening our ties to Canadian industry.