Ultra centrifuge for extraction of high value biomolecules

所要求的Micro-Ultra离心机在亚细胞颗粒、病毒、DNA和RNA的造粒、蛋白质的纯化和分离、脂蛋白的差异浮选、细胞器（核糖体、线粒体、微粒体）和纳米颗粒的分离方面非常有效。这个单位对博士来说非常重要。Ghaly, Brooks, Budge, Ghanem， Gill和Yildiz研究小组。目前的设备已有46年的历史，处于最后阶段，大部分时间都处于停机状态，不能处理小体积，也不能实现小分子分离所需的高速度。该装置将为高价值保健品（鱼油、omega-3脂肪酸、蛋白质、氨基酸）药物（生物活性肽、jadomycin、乳糜蛋白酶、胃蛋白酶、胶原酶、酶抑制剂：α -1抗蛋白酶、α -2巨球蛋白、抗凝血剂：肝素和肝素样糖胺聚糖）的分离提供充足的离心力。酶抑制剂（α -1抗蛋白酶、α -2巨球蛋白）和抗凝血剂（肝素和肝素样糖胺聚糖）的提取及其纯化和回收只有在较高的离心力下才能实现。从鱼血中分离乳糜微粒（用于测量特定脂肪酸的稳定碳同位素）或提取和纯化由天然生物聚合物壳聚糖、细菌细胞中的抗生素（jadomycin）和腺苷和类黄酮的纳米胶囊制成的纳米颗粒是不可能的。将分离鱼废物和藻类中的脂质，以确定鱼油和生物燃料的理论产量，这将决定鱼油生产所需的鱼废物量和微藻生产操作的规模。拟议的工具将用于若干研究项目，这些项目利用可再生自然资源（藻类）、废物（鱼类和动物饲料）生产高附加值产品（生物柴油、营养品、药品），并减轻与处理这些废物有关的环境问题，从而提高当前工业的可持续性，创造新的工业和新的就业机会，并使加拿大在环境保护方面走在前列。生物能源和生物医学技术。

The requested Micro-Ultra Centrifuge is very effective in pelletizing subcellular particles, viruses, DNA and RNA, protein purification and isolation, differential flotation of lipoproteins, isolation of cellular organelles (ribosomes, mitochondria, microsomes) and nanoparticles. The unit is very crucial for Drs. Ghaly, Brooks, Budge, Ghanem, Gill and Yildiz research groups. The current unit is 46 years old and on its last leg, is down most of the time and does not handled small volumes nor the required high speed for the separation of small molecules. The proposed unit will provide ample centrifugal force for separation of high value nutraceuticals (fish oil, omega-3 fatty acids, proteins, aminoacids) pharmaceuticals (bioactive peptides, jadomycin, chymotrypsin, pepsin, collagenase, enzyme inhibitors: alpha-1 antiproteinase, alpha-2 macroglobulin, anticoagulants: heparin and heparin-like glycosaminoglycans). Extraction of enzyme inhibitors (alpha-1 antiproteinase, alpha-2 macroglobulin) and anticoagulants (heparin and heparin-like glycosaminoglycans) and their purification and recovery can only be possible at higher centrifugal force. It is impossible to carry out the separation of chylomicrons from other lipoproteins in fish blood (for measuring the stable carbon isotopes of specific fatty acids) or to extract and purify the nanoparticles formulated from the natural biopolymer chitosan, antibiotics (jadomycin) from bacteria cells and nanoencapsulation of adenosine and flavonoids. Lipids from fish waste and algae will be separated for determining the theoretical fish oil and biofuel yield which will determine the amount of fish waste required for fish oil production and the size of microalgae production operation. The proposed instrument will be use in several research projects which utilize renewable natural resources (algae), waste (fish and animal rendering) to produce high value added products (biodiesel, nutraceuticals, pharmaceuticals) and mitigate environmental problems associated with the disposal of these wastes, thereby improving the sustainability of current industries, creating new industries and new employment opportunities and putting Canada in the fore front of environmental, bioenergy and biomedical technology.