Freeze Dryer for extraction of high value biomolecules

用于提取高价值生物分子的冷冻干燥机

• 批准号: 458547-2014
• 负责人: Ghaly, Abdel
• 金额: $ 2.73万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=541965
• 关键词: Freeze; Dryer; extraction; value; biomolecules

The Freeze Dryer (Labconco Freezone Plus 2.5 L Cascade) is very effective in drying, concentration and evaporation of various samples (milk, urea, anti-cancer drug, antibiotics, nutratceuticals, fish samples, plant samples, fungi and bacteria), evaporation of various solvents, peptide extraction, drying of various polymers and nanoparticles. The requested Freeze Dryer is crucial for the research groups of Dr. Ghaly, Dr. Brooks, Dr. Budge, Dr. Ghanem, Dr. Gill and Dr. Truelstrup Hansen. It will be also used by Dr. Al Taweel, Dr. Mazzanti and Dr. Yildiz. The currently available unit on which the groups relied upon to prepare concentrates for experiments on fractionation and bio-assays for years is a second-hand (20 years old) obsolete freeze dryer. The pump on this old freeze dryer is also second hand (30 years old) and as such requires constant attention and expensive repair and maintenance. The vacuum produced is inadequate for our drying requirements. Thus, the proposed equipment is essential for the current and future research projects on drying blueberries, haskap berries, fish and fish processing waste samples, various nanoparticles, bacteria and fungi and separation and extraction of various nutraceuticals (fish oil, omega-3 fatty acids, proteins, aminoacids) and pharmaceuticals. (bioactive peptides (T2D), jadomycin, chymotrypsin, pepsin, collagenase, enzyme inhibitors: alpha-1 antiproteinase, alpha-2 macroglobulin, anticoagulants: heparin and heparin-like glycosaminoglycans). It is impossible to carry out the separation of chylomicrons from the other lipoproteins in fish blood (for measuring the stable carbon isotopes of specific fatty acids) without freeze dryer. The requested freeze dryer is needed in order to separate/extract and purify the nanoparticles formulated from the natural biopolymer chitosan and antibiotics (jadomycin) from bacteria cells. This equipment will be utilized for training of 52 HQP (7 undergraduate students, 33 graduate students, 2 PDF and 10 technicians).

冷冻干燥机（Labconco Freezone Plus 2.5 L Cascade）在各种样品（牛奶、尿素、抗癌药物、抗生素、营养品、鱼类样品、植物样品、真菌和细菌）的干燥、浓缩和蒸发、各种溶剂的蒸发、肽提取、各种聚合物和纳米颗粒的干燥方面非常有效。所要求的冷冻干燥机对于Ghaly博士、布鲁克斯博士、Budge博士、Ghanem博士、Gill博士和Truelstrup汉森博士的研究小组至关重要。Al Taweel博士、Mazzanti博士和Yildiz博士也将使用该系统。这些研究组多年来用来制备分馏和生物测定实验用浓缩物的现有设备是一台二手（20年前）过时的冷冻干燥机。这个旧的冷冻干燥机上的泵也是二手的（30年），因此需要不断的关注和昂贵的维修和维护。产生的真空度不足以满足我们的干燥要求。因此，所提出的设备对于当前和未来的研究项目至关重要，这些研究项目涉及蓝莓，haskap浆果，鱼类和鱼类加工废物样品，各种纳米颗粒，细菌和真菌以及各种营养品（鱼油，omega-3脂肪酸，蛋白质，氨基酸）和药物的分离和提取。（生物活性肽（T2 D）、雅多霉素、胰凝乳蛋白酶、胃蛋白酶、胶原酶，酶抑制剂：α-1抗蛋白酶、α-2巨球蛋白，抗凝剂：肝素和肝素样糖胺聚糖）。在没有冷冻干燥机的情况下，不可能将乳糜微粒从鱼血液中的其他脂蛋白中分离出来（用于测量特定脂肪酸的稳定碳同位素）。需要所要求的冷冻干燥机，以便从细菌细胞中分离/提取和纯化由天然生物聚合物壳聚糖和抗生素（雅多霉素）配制的纳米颗粒。这些设备将用于培训52名HQP（7名本科生、33名研究生、2名PDF和10名技术员）。