Structure and dynamics of membrane lipids and proteins and their interactions

静态固态核磁共振利用生物膜内有限的分子迁移率来获得组成膜的分子的详细结构和动力学信息。由于固体核磁共振应用于生物分子固体和膜的研究仍处于初级阶段，许多研究计划都指向开发新的见解，以及新的方法和途径。这项建议描述了一种广泛的方法来了解膜的分子组织、分子动力学和分子结构。讨论了两个主要问题：第一个问题涉及膜筏(流液相共存区域)的存在和性质，第二个问题涉及膜肽和蛋白质的结构和动力学。这两个问题是密切相关的，因为了解膜内的相平衡和分子动力学将有可能为膜蛋白的结构研究设计最佳的核磁共振实验。还讨论了胆固醇和多不饱和脂类在确定膜性质中的作用。

Static solid state NMR takes advantage of the restricted molecular mobility within biological membranes to obtain detailed structural and dynamical information about the molecules which make up the membrane. Since the field of solid state NMR applied to biomolecular solids and membranes is still in its infancy, much of the research program is directed towards development of new insights, as well as new methods and approaches. This proposal describes a broad approach to understanding molecular organization, molecular dynamics and molecular structure of membranes. Two principle questions are addressed: the first deals with the existence and properties of membrane rafts (regions of fluid-fluid phase coexistence) and the second with membrane peptide and protein structure and dynamics. These two questions are intimately related since an understanding of the phase equilibria and molecular dynamics within the membrane will make it possible to design optimal NMR experiments for structural studies of membrane proteins. The roles of cholesterol and polyunsaturated lipids in defining the properties of membranes are also addressed.